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Mouse MFRP Antibody

Catalog #: AF3445
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3445
AF3445-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MFRP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MFRP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) MFRP is observed, 15% cross-reactivity with recombinant mouse (rm) Frizzled-4 is observed, 10% cross-reactivity with rmFrizzled-1, rhFrizzled-5, rmFrizzled-8, and rmFrizzled-10 is observed, and 5% cross-reactivity with rmFrizzled-3, rmFrizzled-6, and rmFrizzled-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MFRP
Gln91-Pro584
Accession # Q8K480
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MFRP
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (cerebellum and medulla)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFRP

MFRP is a type II transmembrane protein that is related to a small family of soluble frizzled-related proteins. It is the product of a dicistronic mRNA transcript that codes for two proteins; MFRP and C1qTNF5. Mouse MFRP is 584 amino acids (aa) in length. Its extracellular domain (ECD) of 494 aa contains two CUB and two LDLR class A segments, plus one frizzled domain. At least two splice variants are known in mouse. In isoform 2, amino acid residues 425‑431 of isoform 1 is substituted with Lys. Mouse MFRP ECD shares 72% aa identity with human MFRP ECD. In mouse retinal degeneration 6, MFRP is mutated.

Long Name
Membrane Frizzled-related Protein
Entrez Gene IDs
83552 (Human); 259172 (Mouse)
Alternate Names
MCOP5; membrane frizzled-related protein; Membrane-type frizzled-related protein; MFRP; MGC32938; NNO2; rd6

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Citations for Mouse MFRP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Gene Therapy for Retinitis Pigmentosa Caused by MFRP Mutations: Human Phenotype and Preliminary Proof of Concept
    Authors: Astra Dinculescu, Jackie Estreicher, Juan C. Zenteno, Tomas S. Aleman, Sharon B. Schwartz, Wei Chieh Huang et al.
    Human Gene Therapy
  2. ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrprd6 mouse
    Authors: VM Sluch, A Banks, H Li, MA Crowley, V Davis, C Xiang, J Yang, JT Demirs, J Vrouvliani, B Leehy, S Hanks, AM Hyman, J Aranda, B Chang, CE Bigelow, DS Rice
    Sci Rep, 2018-09-25;8(1):14339.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Spatial and temporal expression of MFRP and its interaction with CTRP5.
    Authors: Mandal MN, Vasireddy V, Jablonski MM
    Invest. Ophthalmol. Vis. Sci., 2006-12-01;47(12):5514-21.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Electron Microscopy, IHC-Fr, Western Blot

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