Mouse MEPE/OF45 Antibody

Catalog #: AF6150 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6150
AF6150-SP

Detection of Mouse MEPE/OF45 by Western Blot.

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All MEPE/OF45 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MEPE/OF45 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MEPE/OF45 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MEPE/OF45 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MEPE/OF45
Ala17-Asp433
Accession # AAK70342

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1.0 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse MEPE/OF45 by Western Blot. Western blot shows lysates of ST-2 mouse bone marrow-derived stromal cell line and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MEPE/OF45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6150) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEPE/OF45 at approximately 58 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEPE/OF45

MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56‑58 kDa member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2 kDa ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417 amino acids (aa) in length. It contains an RGD motif and ASARM domain (aa 416‑433). In addition to its a 57 kDa form, 70 kDa, 42‑45 kDa and 30‑35 kDa forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa 147‑433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.

Long Name

Matrix Extracellular Phosphoglycoprotein with ASARM Motif

Entrez Gene IDs

56955 (Human); 94111 (Mouse); 79110 (Rat)

Alternate Names

extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein; MEPE; OF45; Osteoregulin