Mouse MDL-1/CLEC5A Antibody

Catalog #: MAB1639
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1639
MAB1639-SP

All MDL-1/CLEC5A Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MDL-1/CLEC5A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MDL-1/CLEC5A. In ELISAs, approximately 50% cross-reactivity with recombinant human MDL‑1 is observed.

Source

Monoclonal Rat IgG_2A Clone # 226402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with mouse MDL-1/CLEC5A

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

RAW 264.7 mouse monocyte/macrophage cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDL-1/CLEC5A

MDL-1 is an approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It contains a charged lysine in the transmembrane region that enables it to associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses. The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region and one C-type lectin domain. Within the ECD, mouse and human MDL-1 share 67% amino acid sequence identity. In mouse, alternative splicing generates a short isoform with a 25 aa deletion in the stalk region.

Long Name

Myeloid DAP12-associating Lectin

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Alternate Names

CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse MDL-1/CLEC5A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Cutting Edge: CLEC5A Mediates Macrophage Function and Chronic Obstructive Pulmonary Disease Pathologies
Authors: BW Wortham, BL Eppert, JL Flury, SM Garcia, WR Donica, A Osterburg, B Joyce-Shai, DJ Cua, MT Borchers
J. Immunol., 2016-02-29;196(8):3227-31.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC, IHC-P
Inflammatory arthritis increases mouse osteoclast precursors with myeloid suppressor function.
Authors: Charles, Julia F, Hsu, Lih-Yun, Niemi, Erene C, Weiss, Arthur, Aliprantis, Antonios, Nakamura, Mary C
J Clin Invest, 2012-11-01;122(12):4592-605.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Comparison of gene expression profiles between human and mouse monocyte subsets.
Authors: Ingersoll MA, Spanbroek R, Lottaz C
Blood, 2009-11-12;115(3):e10-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry