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Mouse M-CSF Antibody

Catalog #: AF416
6 Citations Datasheet / COA / SDS
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AF416
AF416-SP
Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse M-CSF Antibody Summary

Specificity
Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human M‑CSF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse M-CSF
Lys33-Glu262
Accession # Q3U4F9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse M-CSF (Catalog # 416-ML)
Neutralization
Measured by its ability to neutralize M‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line [Halenbeck, R. et al. (1989) Biotechnology 7:710]. The Neutralization Dose (ND50) is typically 0.015-0.035 µg/mL in the presence of 10 ng/mL Recombinant Mouse M‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. View Larger

Cell Proliferation Induced by M‑CSF and Neutralization by Mouse M‑CSF Antibody. Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse M-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF416). The ND50 is typically 0.015-0.035 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

References
  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.
Long Name
Macrophage Colony Stimulating Factor
Entrez Gene IDs
1435 (Human); 12977 (Mouse)
Alternate Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

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Citations for Mouse M-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain
    Authors: A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat
    Progress in neurobiology, 2023-01-13;0(0):102405.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Diet-induced inflammation in the anterior paraventricular thalamus induces compulsive sucrose-seeking
    Authors: J Cheng, X Ma, C Li, R Ullah, X Wang, J Long, Z Yuan, S Liu, J Fu, Z Chen, Y Shen, YD Zhou
    Nature Neuroscience, 2022-08-01;25(8):1009-1013.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages
    Authors: I De, V Maklakova, S Litscher, MM Boyd, LC Klemm, Z Wang, C Kendziorsk, LS Collier
    Journal of Neuroinflammation, 2021-07-19;18(1):162.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain
    Authors: LJ Zhou, J Peng, YN Xu, WJ Zeng, J Zhang, X Wei, CL Mai, ZJ Lin, Y Liu, M Murugan, UB Eyo, AD Umpierre, WJ Xin, T Chen, M Li, H Wang, JR Richardson, Z Tan, XG Liu, LJ Wu
    Cell Rep, 2019-06-25;27(13):3844-3859.e6.
    Species: Mouse
    Sample Types: In Vivo, Tissue Homogenates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Neutralization, Western Blot
  5. Microglial pannexin-1 channel activation is a spinal determinant of joint pain
    Authors: M Mousseau, NE Burma, KY Lee, H Leduc-Pess, CHT Kwok, AR Reid, M O'Brien, B Sagalajev, JA Stratton, N Patrick, PL Stemkowski, J Biernaskie, GW Zamponi, P Salo, JJ McDougall, SA Prescott, JR Matyas, T Trang
    Sci Adv, 2018-08-08;4(8):eaas9846.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
    Authors: Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
    Biochem. Biophys. Res. Commun., 2007-06-12;360(2):346-51.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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