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Mouse Lymphotoxin beta R/TNFRSF3 Antibody

Catalog #: AF1008
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1008
AF1008-SP
Detection of Lymphotoxin beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Lymphotoxin beta R/TNFRSF3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Lymphotoxin beta R/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3
Ser28-Pro218
Accession # P50284
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Lymphotoxin  beta R/TNFRSF3
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Lymphotoxin beta R/TNFRSF3 antibody in NIH-3T3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Lymphotoxin beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta R is a member of the TNF receptor superfamily and is now designated TNFRSF3. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric Lymphotoxins LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

Long Name
Lymphotoxin beta Receptor
Entrez Gene IDs
4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)
Alternate Names
CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III

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Citations for Mouse Lymphotoxin beta R/TNFRSF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice
    Authors: L Marrero, J Simkin, M Sammarco, K Muneoka
    Regeneration (Oxf), 2017-05-03;4(2):69-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A dendritic-cell-stromal axis maintains immune responses in lymph nodes.
    Authors: Kumar V, Dasoveanu D, Chyou S, Tzeng T, Rozo C, Liang Y, Stohl W, Fu Y, Ruddle N, Lu T
    Immunity, 2015-04-21;42(4):719-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.
    Authors: Katakai T, Kondo N, Ueda Y, Kinashi T
    J Immunol, 2014-06-16;193(2):617-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009-09-14;119(10):2954-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways.
    Authors: Suto H, Katakai T, Sugai M, Kinashi T, Shimizu A
    Int. Immunol., 2009-02-27;21(4):467-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008-11-01;181(9):6189-200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  7. Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes.
    Authors: Katakai T, Hara T, Sugai M, Gonda H, Shimizu A
    J. Exp. Med., 2004-09-20;200(6):783-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay

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