Mouse LAG-3 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB33281N Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB33281N
Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry.
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Mouse LAG-3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.
Source
Monoclonal Rat IgG2B Clone # 631501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse LAG-3
Ser23-Leu442
Accession # Q61790
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LAG-3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG-3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB33281N, filled histogram) or isotype control antibody (IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of LAG‑3 in CTLL‑2 mouse cytotoxic T cell line by Flow Cytometry CTLL‑2 mouse cytotoxic T cell line were stained with Rat Anti-Mouse LAG‑3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB33281N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Long Name
Lymphocyte-activation Gene 3
Entrez Gene IDs
3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)
Alternate Names
CD223 antigen; CD223; LAG3; LAG-3; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG-3

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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