Mouse L1CAM Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of L1CAM in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B).
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Detection of L1CAM in Neuro-2A cells by Flow Cytometry. Neuro-2A cells were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: L1CAM
L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). L1CAM is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM (2). Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail (3). Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins (4). L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub (4). The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis (1). Overexpression promotes tumor cell invasion and motility, growth in nude mice, and tumor metastasis (1). Proteolytic processing by ADAM10 and
presenilin/ gamma -secretase is essential for the nuclear signaling of L1CAM in human carcinoma cell lines (1). Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome (5, 6).
- Riedle, S. et al. (2009) Biochem. J. 420:391.
- Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
- Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
- Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
- Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
- Striha, L. et al. (2000) J. Child Neurol. 15:239.
Product Datasheets
Citations for Mouse L1CAM Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Viral delivery of L1CAM promotes axonal extensions by embryonic cerebral grafts in mouse brain
Authors: Ryosuke Tsuchimochi, Keitaro Yamagami, Naoko Kubo, Naoya Amimoto, Fabian Raudzus, Bumpei Samata et al.
Stem Cell Reports
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Proteomic and functional analyses of the periodic membrane skeleton in neurons
Authors: R Zhou, B Han, R Nowak, Y Lu, E Heller, C Xia, AH Chishti, VM Fowler, X Zhuang
Nature Communications, 2022-06-09;13(1):3196.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Anandamide-Modulated Changes in Metabolism, Glycosylation Profile and Migration of Metastatic Melanoma Cells
Authors: A Sobiepanek, M Milner-Kra, P Musolf, T Starecki, T Kobiela
Cancers, 2022-03-10;14(6):.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Capture -
L1CAM Is a Marker for Enriching Corticospinal Motor Neurons in the Developing Brain
Authors: B Samata, R Takaichi, Y Ishii, K Fukushima, H Nakagawa, Y Ono, J Takahashi
Front Cell Neurosci, 2020-02-19;14(0):31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Salt-sensitive transcriptome of isolated kidney distal tubule cells
Authors: Elizabeth A. Swanson, Jonathan W. Nelson, Sophia Jeng, Kayla J. Erspamer, Chao-Ling Yang, Shannon McWeeney et al.
Physiological Genomics
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ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM
Authors: Yijian Li, Shujia Huo, Yajie Fang, Ting Zou, Xianliang Gu, Qin Tao et al.
Frontiers in Cellular Neuroscience
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Promotion of spinal cord regeneration by neural stem cell-secreted trimerized cell adhesion molecule l1.
Authors: He, Xiaowen, Knepper, Michael, Ding, Cheng, Li, Jun, Castro, Suita, Siddiqui, Maham, Schachner, Melitta
PLoS ONE, 2012-09-25;7(9):e46223.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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