Mouse Integrin alpha 7 Fluorescein-conjugated Antibody

Catalog #: FAB3518F
2 Citations Datasheet / COA / SDS

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FAB3518F

Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry.

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Citations (2)

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Mouse Integrin alpha 7 Fluorescein-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 7 in direct ELISAs. Reactivity with other Integrin alpha 7 isoforms was not tested. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 2, alpha 3, alpha 4, alpha 5, alpha 9, alpha E, alpha X, recombinant human Integrin alpha L, or alpha 11 is observed.

Source

Monoclonal Rat IgG_2B Clone # 334908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 7
Phe34-Pro1036
Accession # NP_032424

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 7 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Integrin a7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3518F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 7

Integrin alpha 7 is a transmembrane glycoprotein that forms heterodimers with Integrin beta 1 to bind Laminin 1.

Entrez Gene IDs

3679 (Human); 16404 (Mouse); 81008 (Rat); 102130741 (Cynomolgus Monkey)

Alternate Names

FLJ25220; integrin alpha 7 chain; Integrin alpha 7; integrin alpha-7; integrin, alpha 7; ITGA7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Integrin alpha 7 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A role for Galgt1 in skeletal muscle regeneration.
Authors: Singhal N, Martin P
Skelet Muscle, 2015-01-27;5(0):3.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells.
Authors: Fornaro, Mara, Hinken, Aaron C, Needle, Saul, Hu, Erding, Trendelenburg, Anne-Ulr, Mayer, Angelika, Rosenstiel, Antonia, Chang, Calvin, Meier, Viktor, Billin, Andrew N, Becherer, J David, Brace, Arthur D, Evans, William, Glass, David J, Russell, Alan J
Am J Physiol Endocrinol Metab, 2013-11-19;306(2):E150-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay