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Mouse IL-28A/IFN-lambda 2 Biotinylated Antibody

Catalog #: BAF4635
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BAF4635
Product Details
Citations (1)
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Mouse IL-28A/IFN-lambda 2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑28A/IFN‑ lambda 2 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse IL-28B is observed, less than 10% cross-reactivity with recombinant human (rh) IL-28B is observed, and less than 5% cross-reactivity with rhIL-28A is observed.
Source
Polyclonal Sheep IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑28A/IFN-lambda 2 (Catalog # 4635-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-28A/IFN-lambda 2

IL-28A (also named interferon-lambda 2, IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1‑4). They are distantly related to members of the IL-10 family and type I IFN family (1‑4). Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species (5). Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2‑6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor  beta (IL-10 R beta ) and IL-28 receptor  alpha (IL-28 R alpha ; also called IFN-lambda  R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1‑3). Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression (2‑6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9‑11).

References
  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
  5. Ank, N. et al. (2006) J. Virol. 80:4501.
  6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
  7. Siebler, J. et al. (2007) Gastroenterology 132:358.
  8. Meager, A. et al. (2005) Cytokine 31:109.
  9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
Long Name
Interleukin 28A
Entrez Gene IDs
282616 (Human)
Alternate Names
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2

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Citation for Mouse IL-28A/IFN-lambda 2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs.
    Authors: Iversen MB, Ank N, Melchjorsen J, Paludan SR
    J. Virol., 2010-02-24;84(9):4579-86.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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