Mouse IL-23R Antibody

Catalog #: AF1686
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1686
AF1686-SP

All IL-23R Antibodies

Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-23R Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-23 R in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human IL‑23 R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
Gly24-Asp372
Accession # Q8K4B4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑23 R Fc Chimera (Catalog # 1686-MR)

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 40 ng/mL of Recombinant Mouse IL-23 (Catalog # 1887-ML) to immobilized Recombinant Mouse IL-23 R Fc Chimera (Catalog # 1686-MR) coated at 5 µg/mL (100 µL/well). At 30 µg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4⁺ CD45RB^low memory T cells, but at much lower levels by mouse CD4⁺ CD45RB^high cells. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from IL-12.

References

Oppmann, B. et al. (2000) Immunity 13:715.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Parham, C. et al. (2002) J. Immunol. 168:5448.
Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23 Receptor

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Alternate Names

IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-23R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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EBV-induced gene 3 augments IL-23R&alpha protein expression through a chaperone calnexin
Authors: I Mizoguchi, M Ohashi, H Hasegawa, Y Chiba, N Orii, S Inoue, C Kawana, M Xu, K Sudo, K Fujita, M Kuroda, SI Hashimoto, K Matsushima, T Yoshimoto
J. Clin. Invest., 2020-11-02;0(0):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
Clinical and Histopathological Amelioration of Experimental Autoimmune Encephalomyelitis by AAV Vectors Expressing a Soluble Interleukin-23 Receptor
Authors: M Miralles, H Eixarch, M Tejero, C Costa, K Hirota, AR Castaño, M Puig, G Stockinger, X Montalban, A Bosch, C Espejo, M Chillon
Neurotherapeutics, 2017-10-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity
Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana
J. Immunol., 2017-06-21;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity.
Authors: Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE
J. Immunol., 2007-02-01;178(3):1341-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
IL-23 neutralization protects mice from Gram-negative endotoxic shock.
Authors: Belladonna ML, Vacca C, Volpi C, Giampietri A, Fioretti MC, Puccetti P, Grohmann U, Campanile F
Cytokine, 2006-06-08;34(3):161-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization