Mouse IL-22 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC582P-100UG
IC582P-025UG
Detection of IL‑22 in Mouse Th17 Splenocytes by Flow Cytometry.
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Product Details
Citations (6)
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Mouse IL-22 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.
Source
Monoclonal Rat IgG2A Clone # 140301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-22
Leu34-Val179
Accession # Q9JJY9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Mouse splenocytes treated with plate-bound Rat anti-Mouse CD3 epsilon  monoclonal antibody (Catalog # MAB484, 5 μg/ml) plus Goat anti-Mouse CD28 (Catalog # AF483, 5 μg/ml), Recombinant Human TGF-beta 1 (Catalog # 240-B, 10 ng/ml) Recombinant Mouse IL-23 (Catalog # 1887-ML, 20 ng/ml), Recombinant Mouse IL-6 (Catalog # 406-ML, 40 ng/ml), Recombinant Mouse IL-1 beta (Catalog # 401-ML, 10 ng/ml), and Rat anti-Mouse IFN-gamma (Catalog # MAB485, 10 μg/ml) for 5 days were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (Catalog # FC012) and EL‑4 mouse lymphoblast cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-22 antibody in Mouse Th17 Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑22 in Mouse Th17 Splenocytes by Flow Cytometry. Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554A) and (A) Rat Anti-Mouse IL-22 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC582F) and (A) Rat Anti-Mouse IL-22 PE-conjugated Monoclonal Antibody (IC582P) or (B) isotype control antibody (IC006P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry View Larger

Detection of IL‑22 in EL4 cells by Flow Cytometry. EL4 cells were stained with Rat Anti-Mouse IL‑22 PE‑conjugated Monoclonal Antibody (Catalog # IC582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 146 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References
  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Entrez Gene IDs
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

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Citations for Mouse IL-22 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection
    Sci Rep, 2016-09-21;6(0):32927.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge.
    Authors: Walker C, Hautefort I, Dalton J, Overweg K, Egan C, Bongaerts R, Newton D, Cruickshank S, Andrew E, Carding S
    PLoS ONE, 2013-12-17;8(12):e84553.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
    Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
    J. Immunol., 2012-09-19;189(9):4237-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
    Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S
    Mucosal Immunol, 2012-06-27;6(1):69-82.
    Species: Mouse
    Sample Types: Whole Cells
  5. Distinct Roles of IL-23 and IL-17 in the Development of Psoriasis-Like Lesions in a Mouse Model.
    Authors: Nakajima K, Kanda T, Takaishi M, Shiga T, Miyoshi K, Nakajima H, Kamijima R, Tarutani M, Benson JM, Elloso MM, Gutshall LL, Naso MF, Iwakura Y, DiGiovanni J, Sano S
    J. Immunol., 2011-02-23;186(7):4481-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency.
    Authors: Wahl C, Wegenka UM, Leithauser F, Schirmbeck R, Reimann J
    J. Immunol., 2009-04-15;182(8):4521-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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