Mouse IFN-kappa Antibody

Catalog # Availability Size / Price Qty
AF5206
AF5206-SP
Detection of Mouse IFN-kappa  by Western Blot.
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Product Details
Citations (1)
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Mouse IFN-kappa Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN-kappa in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IFN-kappa
Leu22-Lys199 (Cys174Tyr)
Accession # Q7TSL0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IFN-? antibody by Western Blot. View Larger

Detection of Mouse IFN-kappa by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN-kappa at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western Detection of Mouse IFN-? antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse IFN‑ kappa by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL-4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN-kappa at approximately 40 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-kappa

IFN-kappa (Interferon kappa) is a secreted, nonglycosylated polypeptide that belongs to the IFN-kappa subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-kappa induces TNF-alpha, IL-10 and MCP-1 production by monocytes. Mouse IFN-kappa precursor is 199 amino acids (aa) in length. It contains a 21 aa signal sequence, plus a 178 aa mature region. There are two intrachain disulfide bonds plus a fifth unpaired cysteine. Mature mouse IFN-kappa (aa 22‑199) shares 68% and 30% aa identity with rat and human IFN-kappa, respectively.

Long Name
Interferon kappa
Entrez Gene IDs
56832 (Human); 387510 (Mouse)
Alternate Names
IFNK; IFNkappa; IFN-kappa

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Citation for Mouse IFN-kappa Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity
    Authors: AC Billi, M Gharaee-Ke, J Fullmer, LC Tsoi, BD Hill, D Gruszka, J Ludwig, X Xing, S Estadt, SJ Wolf, SM Rizvi, CC Berthier, JB Hodgin, MA Beamer, MK Sarkar, Y Liang, R Uppala, S Shao, C Zeng, PW Harms, ME Verhaegen, JJ Voorhees, F Wen, NL Ward, AA Dlugosz, JM Kahlenberg, JE Gudjonsson
    JCI Insight, 2019-04-18;4(8):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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