Mouse IFN-alpha 4 Antibody

Catalog #: MAB10259 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10259-100
MAB10259-SP

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All IFN-alpha 4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IFN-alpha 4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IFN-alpha 4 in direct ELISAs. In direct ELISAS, no cross-reactivity with recombinant mouse IFNA-1, -2, -6, -7, -9, -11, -12, -13, -15 or -16 is observed.

Source

Monoclonal Rat IgG_2A Clone # 1020125

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived mouse IFN-alpha 4
Leu23-Glu186
Accession # P07351

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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IFN-alpha 4 in Mouse Spleen. IFN-alpha 4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse IFN-alpha 4 Monoclonal Antibody (Catalog # MAB10259) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 4

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and was shown to be very effective in reducing splenomegaly (7).

References

Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
Fung, K.Y. et al. (2013) Science 339:1088.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
van Pesch, V. et al. (2004) J. Virol. 78:8219.
Yeow, W.S. et al. (1998) J. Immunol. 15:2932.
Gerlach, N. et al. (2009) European J. Immunol. 39:136.

Long Name

Interferon alpha 4

Entrez Gene IDs

3441 (Human); 15967 (Mouse)

Alternate Names

IFNA4; IFNalpha 4; IFN-alpha 4; IFN-alpha-4; IFN-alpha4a; INFA4; interferon alpha-4; Interferon alpha-4B; Interferon alpha-76; Interferon alpha-M1; interferon, alpha 4; MGC142200