Mouse Hemopexin Antibody

Catalog #: AF7007
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7007
AF7007-SP

Detection of Mouse Hemopexin by Western Blot.

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All Hemopexin Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Hemopexin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Hemopexin in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant human Hemopexin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Hemopexin
Ser24-Gln460 (His30Asn)
Accession # Q91X72

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Hemopexin by Western Blot. Western blot shows mouse plasma and lysates of mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse Hemopexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hemopexin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Hemopexin in Mouse Kidney. Hemopexin was detected in immersion fixed frozen sections of adult mouse kidney using Sheep Anti-Mouse Hemopexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7007) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to tubule epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemopexin

HPX (Hemopexin) is a 60-70 kDa secreted member of the hemopexin family of molecules. Although it is principally expressed by hepatocytes, it is also reportedly secreted by macrophages, neurons, astrocytes, renal mesangial cells and fibroblasts. It is considered an acute phase protein, and demonstrates a number of effects. Principally, it binds free and albumin-bound heme, neutralizing its potential for oxidative damage to cell membranes. Heme is removed from HPX internally following binding of the HPX:heme complex to CD91 on hepatocytes and macrophages. HPX also dampens the proinflammatory response of macrophages to TLR2 and TLR4 activation. And HPX also shows serine protease activity which may impact cell surface proteins and ECM. Mature mouse HPX is a 437 amino acid (aa) glycoprotein (aa 24-460). It contains five consecutive HPX-like domains (aa 56-349) that likely impart binding-partner specificity to HPX. There are multiple potential isoform variants. One shows a two aa substitution for aa 134-460, a second contains a deletion of aa 60-100, a third possesses a 14 aa substitution for aa 235-460, while a fourth shows a two aa substitution for aa 153-460. Mature mouse HPX shares 92% and 77% aa identity with rat and human HPX, respectively.

Entrez Gene IDs

3263 (Human); 15458 (Mouse); 58917 (Rat)

Alternate Names

Beta-1B-glycoprotein; FLJ56652; Hemopexin; HPX; HX

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Related Research Areas

Citations for Mouse Hemopexin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice
Authors: J Liu, S Lane, R Lall, M Russo, L Farrell, M Debreli Co, C Curtin, R Araujo-Gut, M Scherrer-C, BH Trachtenbe, J Kim, E Tolosano, A Ghigo, RE Gerszten, A Asnani
Science Advances, 2022-12-23;8(51):eadc9245.
Species: Mouse
Sample Types: Tissue Lysate
Applications: Western Blot
Flavin-Containing Monooxygenase 3 (FMO3) Is Critical for Dioxin-Induced Reorganization of the Gut Microbiome and Host Insulin Sensitivity
Authors: W Massey, LJ Osborn, R Banerjee, A Horak, KK Fung, D Orabi, ER Chan, N Sangwan, Z Wang, JM Brown
Metabolites, 2022-04-18;12(4):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot