Mouse F4/80 APC-conjugated Antibody

Name: Mouse F4/80 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB5580A
Rating: 5 (1 reviews)

Catalog #: FAB5580A
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5580A-100UG

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All F4/80 Antibodies

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Citations (2)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F4/80 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse F4/80/EMR1.

Source

Monoclonal Rat IgG_2A Clone # 521204

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1 Gln28-Gly931 Accession # Q61549

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5580A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Entrez Gene IDs

13733 (Mouse); 316137 (Rat)

Alternate Names

ADGRE1; cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; EMR1 hormone receptor; Emr1; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7L; TM7LN3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse F4/80 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Transgenic Overexpression of Galectin-3 in Pancreatic beta Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33
Authors: N Jovicic, I Petrovic, N Pejnovic, B Ljujic, M Miletic Ko, S Pavlovic, I Jeftic, A Djukic, I Srejovic, V Jakovljevi, ML Lukic
Frontiers in Pharmacology, 2021-11-05;12(0):714683.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Leukocyte Beta-Catenin Expression Is Disturbed in Systemic Lupus Erythematosus
PLoS ONE, 2016-08-22;11(8):e0161682.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC