Mouse Ephrin-B2 Biotinylated Antibody Summary
Arg27-Ala227
Accession # AAA82934
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Ephrin-B2 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). Ephrin-B2 antibody in Rat Brain by Immunohistochemistry (IHC-Fr).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Ephrin-B2_BAF496_Immunohistochemistry_10021.jpg)
Ephrin-B2 in Rat Brain. Ephrin-B2 was detected in perfusion fixed frozen sections of rat brain (trigeminal ganglia) using Goat Anti-Mouse Ephrin-B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
![Ephrin-B2 antibody in Rat Hippocampal Neurons by Immunocytochemistry (ICC). Ephrin-B2 antibody in Rat Hippocampal Neurons by Immunocytochemistry (ICC).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/EphrinB2_BAF496_Immunocytochemistry__Immunofluorescence_17414.jpg)
Ephrin‑B2 in Rat Hippocampal Neurons. Ephrin-B2 was detected in immersion fixed rat hippocampal neurons using Goat Anti-Mouse Ephrin-B2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF496) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-B2
Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Product Datasheets
Citations for Mouse Ephrin-B2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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EphB4 forward signalling regulates lymphatic valve development.
Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
Nat Commun, 2015-04-13;6(0):6625.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Emerging pulmonary vasculature lacks fate specification.
Authors: Schwarz MA, Caldwell L, Cafasso D, Zheng H
Am. J. Physiol. Lung Cell Mol. Physiol., 2008-10-24;296(1):L71-81.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-Fr
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