Mouse EphB6 Antibody Summary
Leu33-Ser587
Accession # O08644
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse EphB6 by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF611) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EphB6 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB6
EphB6, also known as Mep (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. However, it has been found that EphB6 contains substitutions within the kinase domain which results in EphB6 having no kinase activity (4). The ligands which bind EphB6 are unknown (2, 3). However, we have observed that the ephrin-B1 and ephrin-B2 ligands can bind the immobilized receptor in an ELISA-type assay. The extracellular domains of human and mouse EphB6 share 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter] (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
- Gurniak, C.B. and L.J. Berg (1996) Oncogene 13:777.
Product Datasheets
Citations for Mouse EphB6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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A Variant in the Nicotinic Acetylcholine Receptor Alpha 3 Subunit Gene Is Associated With Hypertension Risks in Hypogonadic Patients
Authors: Tao Wu, Yujia Wang, Wei Shi, Bi-Qi Zhang, John Raelson, Yu-Mei Yao et al.
Frontiers in Genetics
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The receptor tyrosine kinase EPHB6 regulates catecholamine exocytosis in adrenal gland chromaffin cells
Authors: W Shi, B Ye, M Rame, Y Wang, D Ciocca, S Reibel Foi, J Peng, S Qi, N Vitale, H Luo, J Wu
J. Biol. Chem., 2020-04-22;0(0):.
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture -
EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells
Authors: W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu
J. Biol. Chem., 2019-03-01;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay -
EPHB6 and testosterone in concert regulate epinephrine release by adrenal gland chromaffin cells
Authors: Y Wang, W Shi, A Blanchette, J Peng, S Qi, H Luo, J Ledoux, J Wu
Sci Rep, 2018-01-16;8(1):842.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
Authors: Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
Eur. J. Immunol., 2012-05-23;42(6):1562-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
Nature, 2011-04-20;473(7347):372-5.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
J. Cell. Sci., 2006-02-01;119(0):559-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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