Mouse E-Selectin/CD62E Antibody

Catalog #: MAB5751
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5751-SP
MAB5751-500
MAB5751-100

Product Details

Citations (4)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse E-Selectin/CD62E Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse E-Selectin in ELISAs and Western blots. Does not cross-react with recombinant human (rh) E‑Selectin, rmL-Selectin, or rhP‑Selectin.

Source

Monoclonal Rat IgG₁ Clone # 96419

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin
Trp22-Pro557
Accession # Q00690

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse E‑Selectin/CD62E Fc Chimera (Catalog # 575-ES)
under non-reducing conditions only

Mouse E-Selectin/CD62E Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse E-Selectin/CD62E Biotinylated Antibody (Catalog # BAM5752)

Standard: Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF (Catalog # 575-ES)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Alternate Names

CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse E-Selectin/CD62E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Immunohistochemical study for the expression of leukocyte adhesion molecules, and FGF23 and ACE2 in P. gingivalis LPS-induced diabetic nephropathy
Authors: K Kajiwara, Y Sawa, T Fujita, S Tamaoki
Bmc Nephrology, 2021-01-06;22(1):3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Glycoengineering of Chimeric Antigen Receptor (CAR) T-cells to Enforce E-selectin Binding
Authors: N Mondal, M Silva, AP Castano, MV Maus, R Sackstein
J. Biol. Chem., 2019-10-18;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Plasmodium gametocytes display homing and vascular transmigration in the host bone marrow
Authors: M De Niz, E Meibalan, P Mejia, S Ma, NMB Brancucci, C Agop-Nerse, R Mandt, P Ngotho, KR Hughes, AP Waters, C Huttenhowe, JR Mitchell, R Martinelli, F Frischknec, KB Seydel, T Taylor, D Milner, VT Heussler, M Marti
Sci Adv, 2018-05-23;4(5):eaat3775.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
Authors: Kato N, Yuzawa Y, Kosugi T
J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot