Mouse Dkk-4 Biotinylated Antibody

Catalog #: BAF3105 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF3105

All Dkk-4 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Dkk-4 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Dkk-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Dkk-4, recombinant mouse (rm) Dkk-1, rmDkk-2, and rmDkk-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dkk-4
Leu19-Ile221 (Arg67Ser, Arg70Ser)
Accession # Q8VEJ3.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Dkk‑4 (Catalog # 3105-DK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-4

Dickkopf related protein 4 (Dkk-4) is a member of the Dickkopf protein family that includes Dkk-1, -2, -3, and -4 and a related protein, Soggy (1). Expression of Dkk-4 has only been shown with sensitive PCR techniques during early embryonic development in mice (2), in differentiated human ES cells (3), or in mice that express dominant-active beta -catenin elevating Wnt signaling in the forebrain (4). Dkk proteins are secreted proteins that are synthesized as precursors with an N-terminal signal peptide; all have two conserved cysteine-rich domains separated by a linker region which contains a potential furin type proteolytic cleavage site. The domains contain 10 cysteines each; prokineticin and colipase families show a configuration of cysteines similar to the second motif (5). Mouse Dkk-4 shows 91%, 72% and 71% amino acid (aa) identity with rat, human and canine Dkk-4, respectively, and 40‑46% aa identity with other mouse Dkk proteins. Dkk-4 is predicted as a 25 kDa protein, but transfection of 293T cells produces a shorter (15‑17 kDa) form containing only the second cysteine-rich domain, as well as longer (30‑32 and 40 kDa) forms that do not appear to be glycosylated or form covalent multimers (1). Of the four Dkk proteins, Dkk-4 is the most like Dkk-1. Both are unequivocal antagonists of the canonical Wnt signaling pathway (1, 6), which is activated by Wnt protein engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (7). Dkk-1 and Dkk-4 antagonize Wnt by direct high-affinity binding to LRP5/6, forming ternary complexes of LRP5/6 with the Kremens protein Krm2. Internalization of the complex is triggered, making LRP5/6 unavailable for interaction with Wnt ligands (6‑9).

Long Name

Dickkopf-4

Entrez Gene IDs

27121 (Human); 234130 (Mouse)

Alternate Names

dickkopf (Xenopus laevis) homolog 4; dickkopf homolog 4 (Xenopus laevis); dickkopf-4; dickkopf-related protein 4; Dkk4; Dkk-4; hDkk-4; MGC129562; MGC129563