Mouse DCC Antibody Summary
Phe32-Asn1097
Accession # P70211
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![](https://resources.rndsystems.com/images/datasheets/af844_mouse-dcc-affinity-purified-polyclonal-ab-immunohistochemistry-21102022151713.jpg)
Detection of DCC in Mouse Embryo Developing Brain. DCC was detected in immersion fixed paraffin-embedded sections of Mouse Embryo Developing Brain using Goat Anti-Mouse DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF844) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DCC
Deleted in colorectal cancer (DCC) was originally identified as a putative tumor suppressor gene that is lost in more than 70% of colorectal cancers. This gene has also been found to be deleted in several different kinds of cancers. DCC encodes a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily. The extracellular domain is composed of four Ig-like domains and six fibronectin type III repeats. Two forms of the protein (the long and the short isoforms) are produced from the same gene by the use of alternative initiation sites. A third isoform that is produced by alternative splicing is expressed only in the embryo. The extracellular domain of mouse DCC shares 97% and 99% amino acid sequence identity with the human and rat DCC extracellular domains, respectively. In adults, DCC is highly expressed in the brain but is also expressed at very low levels in multiple tissues. In the embryo, high levels of expression are detected in the brain and neural tube. DCC has been shown to be a receptor for the netrins that are important for axon guidance. DCC has also been shown to induce apoptosis in the absence of ligand binding and to block apoptosis when engaged by netrin-1. DCC has been shown to be a caspase substrate. The pro-apoptotic effects of DCC were found to be dependent on the proteolytic cleavage of the unoccupied receptor by caspase. It is likely that DCC functions as a tumor-suppressor gene by inducing apoptosis in cells that are not exposed to netrins.
- Fearon, E.R. et al. (1990) Science 247:49.
- Keino-Masu, K. et al. (1996) Cell 87:175.
- Mehlen, P. et al. (1998) Nature 395:801.
- Culotti, J.G. and D.C. Merz (1998) Current Opinion in Cell Biology 10:609.
Product Datasheets
Product Specific Notices
This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.
U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.
Citations for Mouse DCC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord
Authors: SG Varadaraja, JH Kong, KD Phan, TJ Kao, SC Panaitof, J Cardin, H Eltzschig, A Kania, BG Novitch, SJ Butler
Neuron, 2017-04-21;0(0):.
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A tug of war between DCC and ROBO1 signaling during commissural axon guidance
Authors: Brianna Dailey-Krempel, Andrew L. Martin, Ha-Neul Jo, Harald J. Junge, Zhe Chen
Cell Reports
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Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury
Authors: Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou
Experimental Eye Research
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Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells
Authors: Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler
STAR Protocols
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Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
Cell Rep, 2020-11-24;33(8):108425.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
Cell Rep, 2019-03-19;26(12):3298-3312.e4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Developmental Requirement of Homeoprotein Otx2 for Specific Habenulo-Interpeduncular Subcircuits
Authors: N Ruiz-Reig, M Rakotobe, I Bethus, G Le Menn, HI Huditz, H Marie, T Lamonerie, F D'Autréaux
J. Neurosci., 2018-12-28;39(6):1005-1019.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis
Authors: Filip A. Konopacki, Hovy Ho-Wai Wong, Asha Dwivedy, Anaïs Bellon, Michael D. Blower, Christine E. Holt
Open Biology
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Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
Authors: Kuwako K, Kakumoto K, Imai T
Neuron, 2010-08-12;67(3):407-21.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
Authors: Hoang S, Liauw J, Choi M
J. Cereb. Blood Flow Metab., 2008-11-05;29(2):385-97.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Sonic hedgehog promotes the migration and proliferation of optic nerve oligodendrocyte precursors.
Authors: Merchan P, Bribian A, Sanchez-Camacho C, Lezameta M, Bovolenta P, De Castro F
Mol. Cell. Neurosci., 2007-08-01;36(3):355-68.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal.
Authors: Tsai HH, Tessier-Lavigne M, Miller RH
Development, 2003-05-01;130(10):2095-105.
Species: Chicken, Rat
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, Neutralization
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