Mouse CX3CR1 APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A) and Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins.
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Detection of CX3CR1 in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either (A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 degreesC as supplied.
Background: CX3CR1
CX3CR1 (CX 3 C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1 lo monocytes plus smooth muscle and mast cells. CX 3 CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1-32, 91-104, 169-196 and 258-274 collectively, mouse CX 3 CR1 shares 96% and 77% aa identity with rat and human CX 3 CR1, respectively.
Product Datasheets
Citations for Mouse CX3CR1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Prevention of food allergy development and suppression of established food allergy by neutralization of TSLP, IL-25 and IL-33
Authors: MV Khodoun, S Tomar, JE Tocker, YH Wang, FD Finkelman
J. Allergy Clin. Immunol., 2017-05-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
Authors: Bryan Simons
J. Immunol, 2016-11-28;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
Blood, 2015-02-03;125(13):2087-94.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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