Mouse Coagulation Factor III/Tissue Factor Antibody

Catalog #: MAB3178
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3178
MAB3178-SP

All Coagulation Factor III/Tissue Factor Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Coagulation Factor III/Tissue Factor Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2C Clone # 355220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Coagulation Factor III
Ala29-Glu251
Accession # P20352

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Coagulation Factor III/Tissue Factor (Catalog # 3178-PA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 - 28) and the mature chain (residues 29 - 294). As a type I membrane protein, it contains a transmembrane region (residues 252 - 274) and a cytoplasmic tail (residues 275 - 294) (3, 4). The purified recombinant mouse TF corresponds to the ectodomain (residues 29 - 251) and is potent in activating thermolysin-processed recombinant mouse Coagulation Factor VII (R&D Systems, Catalog # 3305‑SE) under the conditions described in the Activity Assay Protocol.

References

Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) Academic Press, San Diego, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Ranganathan, G. et al. (1991) J. Biol. Chem. 266:496.
Hartzell, S. (1989) Mol. Cell. Biol. 9:2567.

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Alternate Names

CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Coagulation Factor III/Tissue Factor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1