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Mouse CLEC4F/CLECSF13 Antibody

Catalog #: AF2784
24 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2784
AF2784-SP
CLEC4F/CLECSF13 in Mouse Liver.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CLEC4F/CLECSF13 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13
Ala65-Gly548
Accession # P70194
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CLEC4F/CLECSF13 (Catalog # 2784-CL)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CLEC4F/CLECSF13 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

CLEC4F/CLECSF13 in Mouse Liver. CLEC4F/CLECSF13 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse CLEC4F/CLECSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2784) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

References
  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Bilzer, M. et al. (2006) Liver Int. 26:1175.
  3. Kuiper, J. et al. (1994) Biochem. J. 299:285.
  4. Accession # P70194.
  5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
  6. Fadden, A.J. et al. (2003) Glycobiology 13:529.
  7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
  8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
  9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.
  10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
  11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.
Long Name
C-type Lectin Domain Family 4, Member F
Entrez Gene IDs
165530 (Human); 51811 (Mouse); 114598 (Rat)
Alternate Names
CLEC4F; CLECSF13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13; C-type lectin 13; C-type lectin domain family 4, member F; Galactose Particle Receptor; KCLR; KCR

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Citations for Mouse CLEC4F/CLECSF13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors
    Authors: Alexandra A Tsitrina, Igor V Krasylov, Dmitry I Maltsev, Irina N Andreichenko, Viktoria S Moskvina, Dmitry N Ivankov et al.
    Glycobiology
  2. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver
    Authors: Anneleen Remmerie, Liesbet Martens, Tinne Thoné, Angela Castoldi, Ruth Seurinck, Benjamin Pavie et al.
    Immunity
  3. The Transcription Factor ZEB2 Is Required to Maintain the Tissue-Specific Identities of Macrophages
    Authors: Charlotte L. Scott, Wouter T’Jonck, Liesbet Martens, Helena Todorov, Dorine Sichien, Bieke Soen et al.
    Immunity
  4. Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles
    Authors: Zheng L, Ling W, Zhu D et al.
    iScience
  5. Enterically derived high-density lipoprotein restrains liver injury through the portal vein
    Authors: Han Y, Onufer E, Huang L et al.
    Science
  6. Myeloid-specific deletion of chitinase-3-like 1 protein ameliorates murine diet-induced steatohepatitis progression
    Authors: Andrea D. Kim, Lin Kui, Benedikt Kaufmann, Sung Eun Kim, Aleksandra Leszczynska, Ariel E. Feldstein
    Journal of Molecular Medicine
  7. Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH
    Authors: Sabine Daemen, Mandy M. Chan, Joel D. Schilling
    STAR Protocols
  8. Knockdown of Hyaluronan synthase 2 suppresses liver fibrosis in mice via induction of transcriptomic changes similar to 4MU treatment
    Authors: Halimani, N;Nesterchuk, M;Tsitrina, AA;Sabirov, M;Andreichenko, IN;Dashenkova, NO;Petrova, E;Kulikov, AM;Zatsepin, TS;Romanov, RA;Mikaelyan, AS;Kotelevtsev, YV;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Long-Term L-Glutamine Treatment Reduces Hemolysis without Ameliorating Hepatic Vaso-Occlusion and Liver Fibrosis in a Mouse Model of Sickle Cell Disease
    Authors: Katoch, O;Ungalara, R;Kaminski, T;Li, Z;Dubey, RK;Burholt, I;Gudapati, S;Pradhan-Sundd, T;
    Biomedicines
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers
    Authors: L Li, L Cui, P Lin, Z Liu, S Bao, X Ma, H Nan, W Zhu, J Cen, Y Mao, X Ma, L Jiang, Y Nie, F Ginhoux, Y Li, H Li, L Hui
    Cell Stem Cell, 2023-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Hepatocyte-derived DPP4 regulates portal GLP-1 bioactivity, glucose production, and its absence alters liver disease progression
    Authors: NA Trzaskalsk, B Vulesevic, MA Nguyen, N Jeraj, E Fadzeyeva, NM Morrow, CA Locatelli, N Travis, AA Hanson, JR Nunes, C O'Dwyer, JN Van der Ve, I Lorenzen-S, R Seymour, SM Pulente, AC Clément, AM Crawley, RL Jacobs, MA Doyle, CL Cooper, KH Kim, MD Fullerton, EE Mulvihill
    JCI Insight, 2023-01-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ICC/IF
  12. NLRP6 Inflammasome Modulates Disease Progression in a Chronic-Plus-Binge Mouse Model of Alcoholic Liver Disease
    Authors: RE Mainz, S Albers, M Haque, R Sonntag, NS Treichel, T Clavel, E Latz, KM Schneider, C Trautwein, T Otto
    Cells, 2022-01-06;11(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. COMMD10 is critical for Kupffer cell survival and controls Ly6Chi monocyte differentiation and inflammation in the injured liver
    Authors: K Cohen, O Mouhadeb, S Ben Shlomo, M Langer, A Neumann, N Erez, I Moshkovits, R Pelet, DJ Kedar, E Brazowski, M Guilliams, HS Goodridge, N Gluck, C Varol
    Cell Reports, 2021-11-16;37(7):110026.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Contrasting functional responses of resident Kupffer cells and recruited liver macrophages to irradiation and liver X receptor stimulation
    Authors: T Ishikiriya, H Nakashima, K Endo-Umeda, M Nakashima, S Ito, M Kinoshita, M Ikarashi, M Makishima, S Seki
    PLoS ONE, 2021-07-23;16(7):e0254886.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice
    Authors: Y Jiang, Y Tang, C Hoover, Y Kondo, D Huang, D Restagno, B Shao, L Gao, J Michael Mc, M Zhou, R Silasi-Man, S McGee, M Jiang, X Bai, F Lupu, C Ruan, JD Marth, D Wu, Y Han, L Xia
    Cell Death and Differentiation, 2021-05-15;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  16. OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis
    Authors: L Verboom, A Martens, D Priem, E Hoste, M Sze, H Vikkula, L Van Hove, S Voet, J Roels, J Maelfait, L Bongiovann, A de Bruin, CL Scott, Y Saeys, M Pasparakis, MJM Bertrand, G van Loo
    Cell Rep, 2020-02-18;30(7):2237-2247.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Characterizing responsive and refractory orthotopic mouse models of hepatocellular carcinoma in cancer immunotherapy
    Authors: C Hage, S Hoves, M Ashoff, V Schandl, S Hört, N Rieder, C Heichinger, M Berrera, CH Ries, F Kiessling, T Pöschinger
    PLoS ONE, 2019-07-10;14(7):e0219517.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Authors: KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee
    Sci Rep, 2018-05-01;8(1):6789.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. A CD209 ligand and a sialidase inhibitor differentially modulate adipose tissue and liver macrophage populations and steatosis in mice on the Methionine and Choline-Deficient (MCD) diet
    Authors: Darrell Pilling, Tejas R. Karhadkar, Richard H. Gomer
    PLOS ONE
  20. Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge
    Authors: Friedrich Felix Hoyer, Kamila Naxerova, Maximilian J. Schloss, Maarten Hulsmans, Anil V. Nair, Partha Dutta et al.
    Immunity
  21. Heterogeneity and chimerism of endothelial cells revealed by single-cell transcriptome in orthotopic liver tumors
    Authors: Qi Zhao, Maria del Pilar Molina-Portela, Asma Parveen, Alexander Adler, Christina Adler, Hock E et al.
    Angiogenesis
  22. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
    Authors: Bonnardel J, T'Jonck W, Gaublomme D et al..
    Immunity.
  23. Single‐cell profiling reveals distinct immune phenotypes that contribute to ischaemia‐reperfusion injury after steatotic liver transplantation
    Authors: Xinyu Yang, Di Lu, Rui Wang, Zhengxing Lian, Zuyuan Lin, Jianyong Zhuo et al.
    Cell Proliferation
  24. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches
    Authors: Guilliams M, Bonnardel J, Haest B Et al.
    Cell

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Reviews for Mouse CLEC4F/CLECSF13 Antibody

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Mouse CLEC4F/CLECSF13 Antibody
By Anonymous on 12/19/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Liver tissue Species: Mouse

green color: clec4f

Immunocytochemistry/Immunofluorescence Mouse CLEC4F/CLECSF13 Antibody AF2784
Mouse CLEC4F/CLECSF13 Antibody
By Anonymous on 09/28/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Liver tissue Species: Mouse

The antibody works very well for IF staining in mouse liver either paraffin and frozen sections.

Immunocytochemistry/Immunofluorescence Mouse CLEC4F/CLECSF13 Antibody AF2784