Home / Cerberus 1 / Mouse Cerberus 1 Antibody

Mouse Cerberus 1 Antibody

Catalog #: MAB1986
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1986
MAB1986-SP
Product Details
Citations (11)
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Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Cerberus 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cerberus 1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Cerberus, recombinant mouse (rm) DAN, or rmGremlin.
Source
Monoclonal Rat IgG2A Clone # 225807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cerberus 1
Asp18-Pro272
Accession # O55233
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cerberus 1 (Catalog # 1986-CB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

References
  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  3. Biben, C. et al. (1998) Dev. Biol. 194:135.
  4. Piccolo, S. et al. (1999) Nature 397:707.
  5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
  6. Tavares, A.T. et al. (2007) Development 134:2051.
  7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
  8. Bouwmeester, T. et al. (1996) Nature 382:595.
  9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
  10. Glinka, A. et al. (1997) Nature 389:517.
  11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
  12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
  13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.
Entrez Gene IDs
9350 (Human); 12622 (Mouse)
Alternate Names
CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951

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Citations for Mouse Cerberus 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation
    Authors: Schumacher, S;Fernkorn, M;Marten, M;Chen, R;Kim, YS;Bedzhov, I;Schröter, C;
    Nature communications
    Species: Human
    Sample Types: Embryo
    Applications: Immunohistochemistry
  2. Distinct pathways drive anterior hypoblast specification in the implanting human embryo
    Authors: Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M;
    Nature cell biology
    Species: Human, Mouse
    Sample Types: Embryo
    Applications: Immunohistochemistry
  3. The shapes of elongating gastruloids are consistent with convergent extension driven by a combination of active cell crawling and differential adhesion
    Authors: de Jong, MA;Adegeest, E;Bérenger-Currias, NMLP;Mircea, M;Merks, RMH;Semrau, S;
    PLoS computational biology
    Species: Mouse
    Sample Types: Organoid
    Applications: Immunohistochemistry
  4. An ex vivo system to study cellular dynamics underlying mouse peri-implantation development
    Authors: Takafumi Ichikawa, Hui Ting Zhang, Laura Panavaite, Anna Erzberger, Dimitri Fabrèges, Rene Snajder et al.
    Developmental Cell
  5. Ventricular, atrial, and outflow tract heart progenitors arise from spatially and molecularly distinct regions of the primitive streak
    Authors: Kenzo Ivanovitch, Pablo Soro-Barrio, Probir Chakravarty, Rebecca A. Jones, Donald M. Bell, S. Neda Mousavy Gharavy et al.
    PLOS Biology
  6. Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro
    Authors: Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan et al.
    Developmental Cell
  7. Basement membrane remodelling regulates mouse embryogenesis
    Authors: C Kyprianou, N Christodou, RS Hamilton, W Nahaboo, DS Boomgaard, G Amadei, I Migeotte, M Zernicka-G
    Nature, 2020-05-06;582(7811):253-258.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO
    Authors: Taroh Kinoshita
    J. Cell Biol., 2016-11-23;215(5):705-718.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Secreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells
    Authors: Hidefumi Iwashita, Nobuaki Shiraki, Daisuke Sakano, Takashi Ikegami, Masanobu Shiga, Kazuhiko Kume et al.
    PLoS ONE
  10. A Secreted BMP Antagonist, Cer1, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development
    Authors: Lijun Chi, Ulla Saarela, Antti Railo, Renata Prunskaite-Hyyryläinen, Ilya Skovorodkin, Shelagh Anthony et al.
    PLoS ONE
  11. The anterior visceral endoderm of the mouse embryo is established from both preimplantation precursor cells and by de novo gene expression after implantation.
    Authors: Torres-Padilla ME, Richardson L, Kolasinska P, Meilhac SM, Luetke-Eversloh MV, Zernicka-Goetz M
    Dev. Biol., 2007-07-03;309(1):97-112.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Mouse Cerberus 1 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: E6.5 mouse embryo fixed in 4% PFA Species: Mouse

E6.5 mouse embryo fixed in 4% PFA was stained with this antibody. DAPI was used for nuclear counterstain. Cerberus1 positive cells were seen in the anterior visceral endoderm.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS-T
Dilution: 1:100

Immunocytochemistry/Immunofluorescence Mouse Cerberus 1 Antibody MAB1986