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Mouse CD5L Antibody

Catalog #: AF2834
2 Citations Datasheet / COA / SDS
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AF2834
AF2834-SP
CD5L in Mouse Spleen.
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Citations (2)
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Mouse CD5L Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD5L in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD5L is observed and less than 1% cross-reactivity with recombinant mouse CD5 is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD5L (Catalog # 2834-CL)
Immunohistochemistry
5-15 µg/mL
 Immersion fixed paraffin-embedded sections of mouse spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

CD5L in Mouse Spleen. CD5L was detected in immersion fixed paraffin-embedded sections of mouse spleen using Goat Anti-Mouse CD5L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2834) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD5L

CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine-rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1‑3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is upregulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4+CD8+ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).

References
  1. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  2. Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5.
  3. Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39.
  4. Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151.
  5. Gebe, J.A. et al. (2000) Immunology. 99:78.
  6. Haruta, I. et al. (2001) J. Biol. Chem. 276:22910.
  7. Kuwata, K. et al. (2003) Am. J. Pathol. 162:837.
  8. Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813.
  9. Arai, S. et al. (2005) Cell Metab. 1:201.
  10. Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086.
  11. Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391.
  12. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.
  13. Tissot, J.D. et al. (2002) Electrophoresis 23:1203.
Long Name
CD5 Antigen-like
Entrez Gene IDs
922 (Human); 11801 (Mouse)
Alternate Names
AIM; API6; apoptosis inhibitor 6; CD5 antigen-like (scavenger receptor cysteine rich family); CD5 antigen-like; CD5 molecule-like; CD5L; CT-2; IgM-associated peptide; PRO229; Spalpha; SP-alpha

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Citations for Mouse CD5L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Macrophage CD5L is a target for cancer immunotherapy
    Authors: L Sanchez-Mo, T Paul, C Martori, J Font-Díaz, L Sanjurjo, G Aran, É Téllez, J Blanco, J Carrillo, M Ito, M Tuttolomon, HJ Ditzel, C Fumagalli, G Tapia, J Sidorova, H Masnou, MA Fernández-, JJ Lozano, C Vilaplana, A Rodriguez-, C Armengol, AF Valledor, L Kremer, MR Sarrias
    EBioMedicine, 2023-04-11;91(0):104555.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Limited proteolysis–mass spectrometry reveals aging-associated changes in cerebrospinal fluid protein abundances and structures
    Authors: Steven R. Shuken, Jarod Rutledge, Tal Iram, Patricia Moran Losada, Edward N. Wilson, Katrin I. Andreasson et al.
    Nature Aging

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