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Mouse CD3 epsilon Antibody

Catalog #: MAB484
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB484-SP
MAB484-500
MAB484-100
Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells.
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Citations (18)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD3 epsilon Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4
Source
Monoclonal Hamster IgG Clone # 145-2C11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
H-2Kb-specific mouse cytotoxic T-lymphocyte
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunoprecipitation
Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374.
 
Cell depletion
Small, M. et al. (1994) J. Immunol. Meth. 167:103.
 
CyTOF-reported
Fienberg, H.G. et al. (2012) Cytometry 81: 467. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
T Cell Stimulation
0.1-0.6 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

T Cell Stimulation Mouse CD3e Antibody Stimulates Cell Proliferation antibody in Mouse T Cells. View Larger

Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster Anti-Mouse CD3e Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.4 The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.1 145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.6, 7

References
  1. Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374.
  2. Portoles, P. et al. (1989) J. Immunol. 142:4169.
  3. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703.
  4. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583.
  5. Small, M. et al. (1994) J. Immunol. Meth. 167:103.
  6. Alegre, M.L. et al. (1991) J. Immunol. 146:1184.
  7. Hendrickson, M. et al. (1995) Transplantation 60:828.
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Mouse CD3 epsilon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Role of IL-23 signaling in the progression of premalignant oral lesions to cancer
    Authors: B Caughron, Y Yang, MRI Young
    PLoS ONE, 2018-04-17;13(4):e0196034.
  2. Amnion Epithelial Cells Promote Lung Repair via Lipoxin A4.
    Authors: Tan JL, Tan YZ, Muljadi R et al.
    Stem Cells Transl Med.
  3. High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells
    Authors: SJ Sidles, Y Xiong, MRI Young, AC LaRue
    Mediators Inflamm., 2019-02-28;2019(0):1648614.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IL-23R Signaling Plays No Role in Myocardial Infarction
    Authors: E Engelowski, NF Modares, S Gorressen, P Bouvain, D Semmler, C Alter, Z Ding, U Flögel, J Schrader, H Xu, PA Lang, J Fischer, DM Floss, J Scheller
    Sci Rep, 2018-11-20;8(1):17078.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking
    Authors: T Savinko, C Guenther, LM Uotila, M Llort Asen, S Yao, S Tojkander, SC Fagerholm
    J. Immunol., 2018-03-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis
    Authors: LT McDonald, SD Johnson, DL Russell, MRI Young, AC LaRue
    PLoS ONE, 2017-07-10;12(7):e0180724.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. mTORC2 controls Th9 polarization and allergic airway inflammation
    Authors: H Chen, L Zhang, P Wang, H Su, W Wang, Z Chu, L Zhang, X Zhang, Y Zhao
    Allergy, 2017-03-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors
    Authors: H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka
    PLoS Pathog, 2017-01-03;13(1):e1006120.
  9. Amnion cell-mediated immune modulation following bleomycin challenge: controlling the regulatory T cell response
    Authors: Jean L Tan, Siow T Chan, Camden Y Lo, James A Deane, Courtney A McDonald, Claude CA Bernard et al.
    Stem Cell Research & Therapy
  10. Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration.
    Authors: Gupta S, Marcel N, Talwar S, Garg M, R I, Perumalsamy L, Sarin A, Shivashankar G
    PLoS ONE, 2012-09-05;7(9):e43718.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2010-09-08;25(1):409-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
    Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
    J. Immunol., 2010-01-18;184(4):2130-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
    Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
    Nat. Immunol., 2008-04-06;9(5):513-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  14. Protection of CD8+ T cells from activation-induced cell death by IL-18.
    Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
    J. Leukoc. Biol., 2007-03-30;82(1):142-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  15. Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
    Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
    J. Immunol., 2006-10-15;177(8):5041-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
    Authors: Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
    J. Immunol., 2005-01-15;174(2):953-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
    Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
    J. Neurosci., 2004-07-07;24(27):6133-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. Superoxide Dismutase 3 Controls the Activation and Differentiation of CD4+T Cells
    Authors: Gaurav Agrahari, Shyam Kishor Sah, Chul Hwan Bang, Yeong Ho Kim, Tae-Yoon Kim
    Frontiers in Immunology

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