Mouse CD177 Antibody Summary
Accession # Q8R2S8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD177 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rabbit Anti-Mouse CD177 Monoclonal Antibody (Catalog # MAB8186) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD177
CD177 is a member of the uPAR/CD59/Ly6 superfamily (1). Mature mouse CD177 is a 796 amino acid (aa) protein that contains four uPAR/Ly6 domains, while human CD177 contains only two. Within common regions, mouse CD177 shares 55% and 77% aa sequence identity with human and rat CD177, respectively. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2-4). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (4, 5). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (6, 7). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).
- Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
- Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
- Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
- Klippel, S. et al. (2002) Blood 100:2441.
- Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
- Temerinac, S. et al. (2000) Blood 95:2569.
- Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
- Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
- Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
- von Vietinghoff, S. et al. (2007) Blood 109:4487.
- Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
- van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.
Product Datasheets
Citations for Mouse CD177 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Caloric restriction leads to druggable LSD1-dependent cancer stem cells expansion
Authors: Pallavi, R;Gatti, E;Durfort, T;Stendardo, M;Ravasio, R;Leonardi, T;Falvo, P;Duso, BA;Punzi, S;Xieraili, A;Polazzi, A;Verrelli, D;Trastulli, D;Ronzoni, S;Frascolla, S;Perticari, G;Elgendy, M;Varasi, M;Colombo, E;Giorgio, M;Lanfrancone, L;Minucci, S;Mazzarella, L;Pelicci, PG;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation
Authors: N Wu, XN Nguyen, L Wang, R Appourchau, C Zhang, B Panthu, H Gruffat, C Journo, S Alais, J Qin, N Zhang, K Tartour, F Catez, R Mahieux, T Ohlmann, M Liu, B Du, A Cimarelli
PLoS Pathog., 2019-10-10;15(10):e1008093.
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