Mouse CD151 PE-conjugated Antibody

Catalog #: FAB4609P Datasheet / COA / SDS

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FAB4609P

Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry.

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Mouse CD151 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD151. Stains mouse CD151 transfectants but not irrelevant transfectants.

Source

Monoclonal Rat IgG_2A Clone # 455807

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse CD151
Met1-Tyr253
Accession # BAA22447

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 PE-conjugated Monoclonal Antibody (Catalog # FAB4609P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD151

CD151 is a 29 kDa palmitoylated glycoprotein in the tetraspanin superfamily and is expressed by endothelial cells, epithelial cells, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha 3/ beta 1, alpha 6/ beta 1, alpha 6/ beta 4, and alpha 7/ beta 1. CD151 modulates cellular adhesion, cell migration, and platelet activation. Mouse CD151 shares 94% - 96% aa sequence identity with human and rat CD151.

Entrez Gene IDs

977 (Human); 12476 (Mouse); 64315 (Rat)

Alternate Names

CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151