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Mouse CD11c Antibody

Catalog #: MAB69501
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB69501
MAB69501-SP
Detection of CD11c in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (5)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11c Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD11c.
Source
Monoclonal Hamster IgG Clone # N418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse spleen dendritic cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
3-25 µg/mL
Immersion fixed paraffin-embedded sections of mouse spleen
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD11c antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD11c in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody (F0120) and Rat Anti-Mouse MHC class II (I-A/I-E) APC-conjugated Monoclonal Antibody (FAB6118A). Quadrant markers were set based on normal hamster IgG control antibody staining.

Immunohistochemistry View Larger

Detection of CD11c in Mouse Spleen. CD11c was detected in immersion fixed paraffin-embedded sections of mouse spleen using Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) at 25 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Mouse Anti-Hamster IgG Antibody (Catalog #MAB011) followed by the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11c

CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse CD11c extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat CD11c, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (Complement iC3b, ICAMs, vWF and Fibrinogen) while others (Osteopontin, Thy-1, Plasminogen, Heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.

References
  1. Metlay, J.P. et al. (1990) J. Exp. Med. 171:1753.
Entrez Gene IDs
3687 (Human); 16411 (Mouse); 64350 (Rat)
Alternate Names
95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6

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Citations for Mouse CD11c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
    Sci Rep, 2016-08-22;6(0):31874.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Developmental origin of lung macrophage diversity
    Authors: Serena Y. S. Tan, Mark A. Krasnow
    Development
  3. In vivo adjuvant-induced mobilization and maturation of gut dendritic cells after oral administration of cholera toxin.
    Authors: Anjuere F, Luci C, Lebens M, Rousseau D, Hervouet C, Milon G, Holmgren J, Ardavin C, Czerkinsky C
    J Immunol, 2004-10-15;173(8):5103-11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: FACS
  4. Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.
    Authors: Leenen P, Radosevic K, Voerman J, Salomon B, Van Rooijen N, Klatzmann D, van Ewijk W
    J Immunol, 1998-03-01;160(5):2166-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Functional comparison of spleen dendritic cells and dendritic cells cultured in vitro from bone marrow precursors.
    Authors: Garrigan K, Moroni-Rawson P, McMurray C, Hermans I, Abernethy N, Watson J, Ronchese F
    Blood, 1996-11-01;88(9):3508-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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