Mouse CD11b/Integrin alpha M Antibody

Catalog #: MAB11241
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11241-100
MAB11241-SP

Detection of Mouse CD11b/Integrin alpha M by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11b/Integrin alpha M Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse CD11b/Integrin alpha M in Western blot.

Source

Monoclonal Rat IgG_2B Clone # 908649

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha M beta 2
Phe17-Asn1105 (Integrin alpha M), Gln24-Asn702 (Integrin beta 2)
Accession # NP_032427 (Integrin alpha M) & P11835 (Integrin beta 2)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD11b/Integrin alpha M by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD11b/Integrin alpha M at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of CD11b/Integrin alpha M in RAW 264.7 Mouse Cell Line by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB11241, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1,2).

References

Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Depletion and activation of microglia impact metabolic connectivity of the mouse brain
Authors: J Gnörich, A Reifschnei, K Wind, A Zatcepin, ST Kunte, P Beumers, LM Bartos, T Wiedemann, M Grosch, X Xiang, MK Fard, F Ruch, G Werner, M Koehler, L Slemann, S Hummel, N Briel, T Blume, Y Shi, G Biechele, L Beyer, F Eckenweber, M Scheifele, P Bartenstei, NL Albert, J Herms, S Tahirovic, C Haass, A Capell, S Ziegler, M Brendel
Journal of Neuroinflammation, 2023-02-24;20(1):47.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Diet-dependent function of the extracellular matrix proteoglycan Lumican in obesity and glucose homeostasis
Authors: G Wolff, AE Taranko, I Meln, J Weinmann, T Sijmonsma, S Lerch, D Heide, AT Billeter, D Tews, D Krunic, P Fischer-Po, BP Müller-Sti, S Herzig, D Grimm, M Heikenwäld, WW Kao, A Vegiopoulo
Mol Metab, 2018-10-23;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot