Mouse CCR2 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB5538R
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5538R-025

Detection of CCR2 in Mouse Splenocytes by Flow Cytometry.

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Citations (2)

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Mouse CCR2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Source

Monoclonal Rat IgG_2B Clone # 475301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse/Rat CCR2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB5538R) or (B) Rat IgG_2BAlexa Fluor 647 Isotype Control (Catalog # IC013R). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with mouse CCR2 and eGFP was stained with either (A) Rat Anti-Mouse/Rat CCR2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB5538R) or (B) Rat IgG_2BAlexa Fluor 647 Isotype Control (Catalog # IC013R). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that binds CCL2, CCL7, CCL8, CCL12 (in mouse only) and CCL13. CCR2 is expressed by multiple hematopoietic cells plus, endothelial cells, fibroblasts, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Alternate Names

C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

Product Datasheets

Product Datasheet

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

Citations for Mouse CCR2 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection
Authors: J Filtjens, A Roger, L Quatrini, E Wieduwild, J Gouilly, G Hoeffel, R Rossignol, C Daher, G Debroas, S Henri, CM Jones, B Malissen, LK Mackay, A Moqrich, FR Carbone, S Ugolini
Nature Communications, 2021-05-18;12(1):2936.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Exosomal MiRNA Transfer between Retinal Microglia and RPE
Authors: DR Morris, SE Bounds, H Liu, WQ Ding, Y Chen, Y Liu, J Cai
Int J Mol Sci, 2020-05-17;21(10):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry