Mouse CCL21/6Ckine Antibody Summary
Ser24-Gly133
Accession # P84444
Applications
Mouse CCL21/6Ckine Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL21/6Ckine
6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid, highly basic, precursor protein with a 23 aa signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid protein with a 23 signal peptide that is cleaved to generate the 110 aa mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% aa sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines.
- Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
- Hromas, R. et al. (1997) J. Immunol. 159:2554.
- Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.
Product Datasheets
Citations for Mouse CCL21/6Ckine Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Endothelial Erg Regulates Expression of Pulmonary Lymphatic Junctional and Inflammation Genes in Mouse Lungs Impacting Lymphatic Transport
Authors: Chakraborty, A;Kim, A;AlAbdullatif, S;Campbell, JD;Alekseyev, YO;Kaplan, U;Dambal, V;Ligresti, G;Trojanowska, M;
Research square
Species: Human hepegivirus, Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense
Authors: FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang
Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
The lymph node stromal laminin alpha 5 shapes alloimmunity
Authors: L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg
J. Clin. Invest., 2020-05-01;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization.
Authors: Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y
Blood, 2006-06-29;108(8):2531-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Thymic T cell development and progenitor localization depend on CCR7.
Authors: Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Forster R
J. Exp. Med., 2004-08-09;200(4):481-91.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
Authors: Ato M, Stager S, Engwerda CR, Kaye PM
Nat. Immunol., 2002-11-18;3(12):1185-91.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Herpes simplex virus (HSV) amplicon-mediated codelivery of secondary lymphoid tissue chemokine and CD40L results in augmented antitumor activity.
Authors: Tolba KA, D†ÃÅê†Ò, Bowers WJ, Muller J, Housekneckt V, Giuliano RE, Federoff HJ, Rosenblatt JD
Îζ)Š4wJ]¾b.ŠKd•ÿ¶fäMò&¨k³, 2002-11-15;62(22):6545-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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