Mouse Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Catalog # Availability Size / Price Qty
MAB1123
MAB1123-SP
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Mouse Cathepsin A/Lysosomal Carboxypeptidase A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse (rm) DPPIV/CD26 is observed. In Western blots, it recognizes the single chain (55 kDa) form of rmCathepsin A, which can be processed into heavy (32 kDa) and light (20 kDa) chains. It recognizes the heavy chain, but not the light chain.
Source
Monoclonal Rat IgG2B Clone # 191430
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin A/Lysosomal Carboxypeptidase A
Ala24-Tyr474 (predicted)
Accession # P16675
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Cathepsin A/Lysosomal Carboxypeptidase A
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Cathepsin A/
Lysosomal Carboxypeptidase A, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1, 2). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal cathepsin A plays a role in regulation of functions of these molecules (3). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

References
  1. Galjart, N.J. et al. (1990) J. Biol. Chem. 265:4678.
  2. Pshezhetsky, A.V. (1998) in Handbook of Proteolytic Enzymes (ed. Barrett, Rawlings, Woessner) pp. 393, Academic Press, San Diego.
  3. Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.
Entrez Gene IDs
5476 (Human); 19025 (Mouse)
Alternate Names
beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; cathepsin ANGBE; CTSA; EC 3.4.16; EC 3.4.16.5; GLB2; GSL; Lysosomal Carboxypeptidase A; lysosomal protective protein; PPCA; PPGBprotective protein for beta-galactosidase (galactosialidosis); Protective protein cathepsin A; Protective protein for beta-galactosidase

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