Mouse/Canine Integrin beta 2/CD18 Antibody

Catalog #: AF2618
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2618
AF2618-SP

Detection of Integrin beta 2/CD18 in Canine PBMCs by Flow Cytometry.

1 Image

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Canine Integrin beta 2/CD18 Antibody Summary

Species Reactivity

Mouse, Canine

Specificity

Detects mouse Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 5% cross‑reactivity with rmIntegrin beta 1, rmIntegrin beta 4, and rmIntegrin beta 7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 2
Gln24-Asn702
Accession # P11835

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Integrin beta 2/CD18

Flow Cytometry

2.5 µg/10⁶ cells

Mouse lymph node cells and canine peripheral blood mononuclear cells (PBMCs)

Adhesion Activation

The adhesion of CD3⁺ purified mouse T cells to immobilized Recombinant Mouse ICAM‑1 Fc Chimera (Catalog # 796-IC, 10 µg/mL, 100 µL/well) was maximally enhanced (2-2.5 fold) by 1 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Adhesion Activation

Detection of Integrin beta 2/CD18 antibody in Canine PBMCs antibody by Flow Cytometry.

View Larger

Detection of Integrin beta 2/CD18 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Mouse/Canine Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2618, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Mouse Integrin beta 2 is a transmembrane glycoprotein with an 85 kDa molecular weight (predicted) and is expressed by all leukocytes. The Integrin beta chain can be found in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4) leading to different Integrin proteins. The extra cellular domains of mouse and canine Integrin beta 2 share an 80.3% aa sequence identity. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling and leukocyte trafficking.

Entrez Gene IDs

3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)

Alternate Names

959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Canine Integrin beta 2/CD18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by: