Mouse BST-2/Tetherin Antibody

Catalog #: MAB8660
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8660
MAB8660-SP

Detection of BST-2/Tetherin in Mouse Bone Marrow Cells by Flow Cytometry.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BST-2/Tetherin Antibody Summary

Specificity

Detects mouse BST-2/Tetherin in flow cytometry.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 44E9R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Recombinant extracellular domain of CD317

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of BST-2/Tetherin in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry

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BST-2/Tetherin in Mouse Splenocytes. BST-2/Tetherin was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BST-2/Tetherin

BST-2, also known as Tetherin or PDCA1 and designated CD317, is a 30-35 kDa interferon-inducible protein that shows an unusual topology. The N-terminus is cytoplasmic, followed by a transmembrane segment, an extracellular loop, and a C-terminal GPI-linkage. BST-2 is expressed on bone marrow stromal cells and is upregulated in breast cancer and astrocytoma. It binds to ILT7 on plasmacytoid dendritic cells and inhibits proinflammatory TLR7 and TLR9 signaling. BST-2 inhibits the release of Kaposi sarcoma virus, HIV-1, and Lassa virus from infected cells, but this function is counteracted by viral proteins which directly bind and trigger the degradation of BST-2.

Long Name

Bone Marrow Stromal Cell Antigen 2

Entrez Gene IDs

684 (Human); 69550 (Mouse)

Alternate Names

bone marrow stromal antigen 2; bone marrow stromal cell antigen 2; BST2; BST-2; CD317 antigen; CD317; HM1.24 Antigen; NPC-A-7; PDCA-1; Tetherin

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Mouse BST-2/Tetherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis
Authors: LC Dorman, PT Nguyen, CC Escoubas, ID Vainchtein, Y Xiao, PV Lidsky, H Nakajo, NJ Silva, C Lagares-Li, EY Wang, SE Taloma, B Cuevas, H Nakao-Inou, BM Rivera, B Schwer, C Condello, R Andino, TJ Nowakowski, AV Molofsky
bioRxiv : the preprint server for biology, 2023-03-20;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IF