Mouse B7-H2 Antibody

Catalog #: MAB1582 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1582-100
MAB1582-SP

Detection of B7‑H2 in HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP by Flow Cytometry.

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Mouse B7-H2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse B7-H2 in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 987614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Met1-Lys279
Accession # Q9JHJ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (50 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with mouse B7-H2. Binding is blocked by 2.5 μg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582).

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of B7‑H2 in HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP were stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) followed by Goat Anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction

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ICOS Binding to B7-H2-transfected HEK293 Cell Line is Blocked by Mouse B7-H2 Antibody. In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (168-CS; 50 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with mouse B7-H2. Binding is blocked by (A) 2.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB1582) but not by (B) Rat IgG1 Isotype Control (MAB005) at 2.5 µg/mL. Protein binding was detected with APC-conjugated anti-Human IgG Fc Monoclonal Antibody (FAB110A).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H2

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Long Name

B7 Homolog 2

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Alternate Names

B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand