Mouse B7-H2 Antibody Summary
rhB7-H3b, rhPD-L2, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H3, rmB7-H4, rmPD-L2, or recombinant rat B7-2 is observed.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of B7‑H2 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Mouse B7-H2 Monoclonal Antibody (Catalog # MAB158, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).
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B7‑H2 in Mouse Splenocytes. B7-H2 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB158) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
Background: B7-H2
B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.
Product Datasheets
Citation for Mouse B7-H2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion
Authors: Guillem Angulo, Jelena Zeleznjak, Pablo Martínez-Vicente, Joan Puñet-Ortiz, Hartmut Hengel, Martin Messerle et al.
eLife
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