Mouse Aminopeptidase PILS/ARTS1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2500
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Mouse Aminopeptidase PILS/ARTS1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Aminopeptidase PILS/ARTS1 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human ARTS1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase PILS/ARTS1
Ser27-Leu930
Accession # NP_109636
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Aminopeptidase PILS/ARTS1 (Catalog # 2500-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase PILS/ARTS1

The mouse ARTS1 gene encodes aminopeptidase PILS (Puromycin-Insensitive Leucyl-Specific), which is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum) (1‑3). The deduced amino acid sequence of mouse ARTS1 consists of a signal peptide and a large ectodomain. Widely expressed and releasing an N‑terminal amino acid (Leu), the enzyme may play a role in many processes such as antigen processing and angiogenesis.

References
  1. Miyashita, H. et al. (2002) Blood 99:3241.
  2. Serwold, T. et al. (2002) Nature 419:480.
  3. Schomburg, L. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 311, Academic Press, San Diego.
Long Name
Aminopeptidase Puromycin-insensitive Leucyl-specific
Entrez Gene IDs
51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)
Alternate Names
Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

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