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Mouse AgRP/ART Antibody

Catalog #: AF634
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF634
AF634-SP
AgRP/ART in Mouse Brain.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse AgRP/ART Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse AgRP in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse AgRP/ART
Val25-Thr131
Accession # P56473
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1.7-5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry AgRP/ART antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

AgRP/ART in Mouse Brain. AgRP/ART was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Goat Anti-Mouse AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF634) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuroprotein that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) action on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature mouse AgRP is a 111 amino acid (aa) polypeptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Within the C-terminal region, mouse AgRP shares 80% and 90% aa sequence identity with human and rat AgRP, respectively. It also shares 44% aa sequence identity with Agouti. As with Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP, however, is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones via a mechanism that does not involve melanocortin receptors (14).

References
  1. Martin, N.M. et al. (2006) Peptides 27:333.
  2. Fan, W. et al. (1997) Nature 385:165.
  3. Ollmann, M.M. et al. (1997) Science 278:135.
  4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
  5. Shutter, J.R. et al. (1997) Genes Dev. 11:593.
  6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
  7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
  8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
  9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
  10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
  11. Small, C.K. et al. (2001) Diabetes 50:248.
  12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
  13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
  14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.
Long Name
Agouti-related Protein
Entrez Gene IDs
181 (Human); 11604 (Mouse)
Alternate Names
agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2

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Citations for Mouse AgRP/ART Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Malocclusion impairs cognitive behavior via AgRP signaling in adolescent mice
    Authors: Junya Kusumoto, Koji Ataka, Haruki Iwai, Yasuhiko Oga, Keita Yamagata, Kanako Marutani et al.
    Frontiers in Neuroscience
  2. Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice
    Authors: Lyvianne Decourtye-Espiard, Maud Clemessy, Patricia Leneuve, Erik Mire, Tatiana Ledent, Yves Le Bouc et al.
    Nutrients
  3. A subpopulation of agouti-related peptide neurons exciting corticotropin-releasing hormone axon terminals in median eminence led to hypothalamic-pituitary-adrenal axis activation in response to food restriction
    Authors: Ruksana Yesmin, Miho Watanabe, Adya Saran Sinha, Masaru Ishibashi, Tianying Wang, Atsuo Fukuda
    Frontiers in Molecular Neuroscience
  4. Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging
    Authors: Ayumu Sugiura, Tatsuhiro Shimizu, Takeshi Kameyama, Tomohiko Maruo, Shin Kedashiro, Muneaki Miyata et al.
    Frontiers in Aging Neuroscience
  5. Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance
    Authors: S Kim, N Kim, S Park, Y Jeon, J Lee, SJ Yoo, JW Lee, C Moon, SW Yu, EK Kim
    Autophagy, 2019-08-30;0(0):1-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism
    Authors: Z Ou, Y Ma, Y Sun, G Zheng, S Wang, R Xing, X Chen, Y Han, J Wang, QR Lu, TJ Zhao, Y Chen
    Cell Rep, 2019-03-12;26(11):2984-2997.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Functional identity of hypothalamic melanocortin neurons depends on Tbx3
    Authors: Carmelo Quarta, Alexandre Fisette, Yanjun Xu, Gustav Colldén, Beata Legutko, Yu-Ting Tseng et al.
    Nature Metabolism
  8. High-fat diet accelerates extreme obesity with hyperphagia in female heterozygous Mecp2-null mice
    Authors: S Fukuhara, H Nakajima, S Sugimoto, K Kodo, K Shigehara, H Morimoto, Y Tsuma, M Moroto, J Mori, K Kosaka, M Morimoto, H Hosoi
    PLoS ONE, 2019-01-04;14(1):e0210184.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei
    Authors: Katie Sokolowski, Tuyen Tran, Shigeyuki Esumi, Yasmin Kamal, Livio Oboti, Julieta Lischinsky et al.
    Neural Development
  10. QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior
    PLoS ONE, 2016-11-11;11(11):e0164716.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
    Authors: Cortes-Campos C, Elizondo R, Carril C, Martinez F, Boric K, Nualart F, Garcia-Robles M
    PLoS ONE, 2013-04-26;8(4):e62532.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC

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