Mouse ACE-2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB34372-100
MAB34372-SP
Detection of Mouse ACE‑2 by Western Blot.
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Product Details
Citations (1)
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Mouse ACE-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ACE-2 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2818I
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived mouse ACE-2
Gln18-Thr740
Accession # NP_081562
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Mouse kidney, mouse ovary, mouse heart, and mouse testis
Flow Cytometry
10 µg/106 cells
HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP
Immunocytochemistry
3-25 µg/mL
Immersion fixed HEK293 human embryonic kidney cell line transfected with ACE-2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Mouse ACE‑2 by Western Blot. Western blot shows lysates of mouse kidney, mouse ovary, mouse heart, and mouse testis. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ACE‑2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry View Larger

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Mouse ACE-2 Monoclonal Antibody (Catalog # MAB34372) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry View Larger

ACE‑2 in HEK293 Human Cell Line transfected with ACE-2. ACE‑2 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with ACE-2 (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection of Mouse ACE-2 by Simple Western View Larger

Detection of Mouse ACE-2 by Simple Western Expression of hACE2 in hACE2-KI mouse model. (A) The expression pattern of hACE2 mRNA in different organs including brain, heart, intestines, kidneys, liver, lungs, spleen, stomach, and trachea was detected by real-time quantitative PCR (qPCR) assay. (B) Representative immunoblotting image shows the expression of the ACE2 protein in mouse intestine and lung tissues normalized to total protein. Human ACE2 was highly expressed in the lungs and intestines of hACE2-KI mice, while mouse ACE2 protein was detected in WT mice but not in hACE2-KI mice. (C) Immunochemistry results of the hACE2 expression in different organs. The intestines, lungs, and trachea sections are shown in high-power magnification; scale bar: 100 μm. Statistical analysis was performed using unpaired Student’s t-test or Wilcoxon rank test. NS: not significant; *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37350787), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

References
  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower, M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Ima, Y. et al. (2005) Nature 436:112.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

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Citation for Mouse ACE-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A novel hACE2 knock-in mouse model recapitulates pulmonary and intestinal SARS-CoV-2 infection
    Authors: Zhou, X;Sun, W;Zhang, Y;Gu, H;Wang, R;Xie, P;Zhu, Y;Qiu, M;Ding, X;Wang, H;Gao, Y;Li, J;
    Frontiers in microbiology
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Simple Western

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