Mouse 4-1BB/TNFRSF9/CD137 Antibody

Name: Mouse 4-1BB/TNFRSF9/CD137 Antibody
Brand: R&D Systems
SKU: MAB9371
Rating: 5 (2 reviews)

Catalog #: MAB9371
6 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9371-SP
MAB9371-100
MAB9371-500

All 4-1BB/TNFRSF9/CD137 Antibodies

Product Details

Citations (6)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse 4-1BB/TNFRSF9/CD137 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse 4-1BB in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse (rm) BAFF R, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmLT beta R, rmNGF R, rmOPG, rmRANK, rmTAJ, rmTNF RI, rmTNF RII, rh4-1BB, rhDR6, or rhHVEM.

Source

Monoclonal Rat IgG_2A Clone # 158321

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
Val24-Leu187
Accession # P20334

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Agonist Activity

Measured by its ability to co-stimulate IFN-gamma secretion by mouse splenic T cells in the presence of anti-CD3 [Saoulli, K. et al. (1998) J. Exp. Med. 187(11):1849; Cannons, J. et al. (2001) J. Immunol. 167:1313.].
The ED₅₀ for this effect is typically 0.4 - 1.2 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is a type I transmembrane protein belonging to the TNF receptor superfamily. It has been designated TNFRSF9 and is also known as CD137. 4-1BB is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector function. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Alternate Names

4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse 4-1BB/TNFRSF9/CD137 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Integrin &alphav&beta8 on Tï¿½cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy
Authors: E Dodagatta-, HY Ma, B Liang, J Li, DS Meyer, SY Chen, KH Sun, X Ren, B Zivak, MD Rosenblum, MB Headley, L Pinzas, NI Reed, JS Del Cid, BC Hann, S Yang, A Giddabasap, K Noorbehesh, B Yang, J Dal Porto, T Tsukui, K Niessen, A Atakilit, RJ Akhurst, D Sheppard
Cell Reports, 2021-07-06;36(1):109309.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Epitope and Fc-mediated Crosslinking, but not High Affinity, Are Critical for Antitumor Activity of CD137 Agonist Antibody with Reduced Liver Toxicity
Authors: SK Ho, Z Xu, A Thakur, M Fox, SS Tan, E DiGiammari, L Zhou, M Sho, B Cairns, V Zhao, M Xiong, J Samayoa, CM Forsyth, DB Powers, DT Chao, D Hollenbaug, HM Alvarez, Y Akamatsu
Mol. Cancer Ther., 2020-01-23;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Agonist Activity
4-1BB Signaling Promotes Alveolar Macrophages-Mediated Pro-Fibrotic Responses and Crystalline Silica-Induced Pulmonary Fibrosis in Mice
Authors: Yiping Lu, Chao Li, Sitong Du, Xi Chen, Xinning Zeng, Fangwei Liu et al.
Frontiers in Immunology
PET imaging to non-invasively study immune activation leading to antitumor responses with a 4-1BB agonistic antibody
Authors: Helena Escuin-Ordinas, Mark W Elliott, Mohammad Atefi, Michelle Lee, Charles Ng, Liu Wei et al.
Journal for ImmunoTherapy of Cancer
Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
Authors: Wells JW, Cowled CJ, Farzaneh F
J. Immunol., 2008-09-01;181(5):3422-31.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
Arthritis Rheum., 2007-03-01;56(3):949-59.
Species: Mouse
Sample Types: In Vivo
Applications: Control