Human XCR1 Antibody

Catalog #: MAB8571 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8571
MAB8571-SP

Detection of XCR1 in CD141+and HLA-DR+Human Blood Monocytes by Flow Cytometry.

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Human XCR1 Antibody Summary

Specificity

Detects human XCR1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1097A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of XCR1 in CD141⁺and HLA-DR⁺Human Blood Monocytes by Flow Cytometry. CD141⁺and HLA-DR⁺human peripheral blood monocytes were isolated by staining with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated Monoclonal Antibody (Catalog # FAB4869F) and Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog # FAB3947A). Isolated cells were then stained with (B) Rabbit Anti-Human XCR1 Monoclonal Antibody (Catalog # MAB8571, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XCR1

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8⁺ T cells, NK cells, CD4⁺ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Long Name

Chemokine [C Motif] Receptor 1

Entrez Gene IDs

2829 (Human); 23832 (Mouse); 301086 (Rat)

Alternate Names

CCXCR1; CCXCR1XC chemokine receptor 1; chemokine (C motif) receptor 1; chemokine XC receptor 1; G protein-coupled receptor 5; GPR5; GPR5chemokine (C motif) XC receptor 1; G-protein coupled receptor 5; Lymphotactin receptor; XCR1