Human Wnt-9a Alexa Fluor® 488-conjugated Antibody Summary
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Wnt‑9a in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Wnt-9a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC3157G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Wnt-9a
Wnt-9a (Wingless integration site family member 9a), formerly Wnt-14, is a 45-47 kDa member of the Wnt family of proteins, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in both embryo and adult. It is found in embryonic mesenchyme adjacent to cartilage, and in lung at the tips of developing bronchioles. In adult, it is found throughout the liver, in hepatocytes, stellate sinusoidal cells, biliary epithelium and endothelium. It also appears in colonic epithelium and in many tumor cell types. It would appear that Wnt-9a binds to Fzd-7 and -9. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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