Human WARP Antibody

Catalog #: MAB6189 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6189
MAB6189-SP

Detection of Human WARP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human WARP Antibody Summary

Species Reactivity

Human

Specificity

Detects human WARP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) WARP, rmCollagen II, or recombinant bovine Collagen I is observed.

Source

Monoclonal Mouse IgG₁ Clone # 559915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WARP
Arg19-Pro445
Accession # Q6PCB0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human WARP by Western Blot. Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human WARP Monoclonal Antibody (Catalog # MAB6189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for WARP at approximately 50, 40 and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WARP

Von Willebrand factor A (vWFA) domain-related protein (WARP) is a 50 kDa glycoprotein member of the vWFA domain superfamily of extracellular matrix proteins. It is expressed in embryonic articular cartilage, skeletal muscle and basement membranes in the PNS. WARP forms disulfide-linked homodimers and multimers, and complexes with perlecan. Secreted human WARP contains a vWFA domain (aa 34‑213), two fibronectin type III domains (aa 211‑301 and 331‑421) that likely bind to the GAG modification of perlecan, and one potential site for N-linked glycosylation. There is one alternate start site at Met213. Mature human WARP shares 72% aa sequence identity with mature mouse and rat WARP.

Long Name

von Willebrand factor A Domain-related Protein

Entrez Gene IDs

64856 (Human); 246228 (Mouse); 298683 (Rat)

Alternate Names

DKFZp761O051; von Willebrand factor A domain containing 1; von Willebrand factor A domain-containing protein 1; von Willebrand factor A domain-related protein; VWA1; WARP