Human VISTA/B7-H5/PD-1H Antibody

Name: Human VISTA/B7-H5/PD-1H Antibody
Brand: R&D Systems
SKU: MAB71261
Rating: 5 (1 reviews)

Catalog #: MAB71261
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB71261
MAB71261-SP

Detection of VISTA/B7-H5/PD-1H in Human PBMCs by Flow Cytometry.

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All VISTA/B7-H5/PD-1H Antibodies

Product Details

Citations (2)

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Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VISTA/B7-H5/PD-1H Antibody Summary

Species Reactivity

Human

Specificity

Detects human VISTA/B7-H5/PD-1H in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse VISTA/B7-H5/PD-1H is observed.

Source

Monoclonal Mouse IgG_2B Clone # 730804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VISTA/B7-H5/PD-1H
Phe33-Ala194
Accession # Q9H7M9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VISTA/B7-H5/PD-1H in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71261) or (B) Mouse IgG_2BIsotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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VISTA/B7-H5/PD-1H in BG01V Human Embryonic Stem Cells. VISTA/B7-H5/PD-1H was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71261) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Alternate Names

4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human VISTA/B7-H5/PD-1H Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Interferon-? converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1
Authors: R Liu, J Merola, TD Manes, L Qin, GT Tietjen, F López-Girá, V Broecker, C Fang, C Xie, PM Chen, NC Kirkiles-S, D Jane-Wit, JS Pober
JCI Insight, 2018-03-08;3(5):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Characterization of programmed death-1 homologue-1 (PD-1H) expression and function in normal and HIV infected individuals.
Authors: Bharaj, Preeti, Chahar, Harendra, Alozie, Ogechika, Rodarte, Lizette, Bansal, Anju, Goepfert, Paul A, Dwivedi, Alok, Manjunath, N, Shankar, Premlata
PLoS ONE, 2014-10-03;9(10):e109103.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC