Home / Versican / Human Versican Isoform V0 Antibody

Human Versican Isoform V0 Antibody

Catalog #: AF3054
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3054
AF3054-SP
Versican in HeLa Human Cell Line.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Versican Isoform V0 Antibody Summary

Specificity
Detects human Versican isoform V0 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Versican Isoform V0
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Versican antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Versican in HeLa Human Cell Line. Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Versican in U‑251 MG Human Cell Line. Versican was detected in immersion fixed U‑251 MG human glioblastoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Versican

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Entrez Gene IDs
1462 (Human); 13003 (Mouse)
Alternate Names
Chondroitin sulfate proteoglycan 2WGN; Chondroitin sulfate proteoglycan core protein 2; CSPG2; CSPG2DKFZp686K06110; ERVR; GHAP; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PG-MWGN1; VCAN; versican core protein; versican proteoglycan; Versican

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Citations for Human Versican Isoform V0 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics
    Authors: Riabinin, A;Kalabusheva, E;Khrustaleva, A;Akulinin, M;Tyakht, A;Osidak, E;Chermnykh, E;Vasiliev, A;Vorotelyak, E;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Reorganization of the Brain Extracellular Matrix in Hippocampal Sclerosis
    Authors: B Sitaš, M Bobi?-Raso, G Mrak, S Trnski, M Krbot Skor, D Oreškovi?, V Knezovi?, Ž Petelin Ga, Z Petanjek, G Šimi?, D Kolenc, N Jovanov Mi
    International Journal of Molecular Sciences, 2022-07-25;23(15):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix
    Authors: S Taylor, M Whitfield, J Barratt, A Didangelos
    J. Immunol., 2020-09-11;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Versican is differentially regulated in the adventitial and medial layers of human vein grafts
    Authors: RD Kenagy, S Kikuchi, SP Evanko, MS Ruiter, M Piola, A Longchamp, M Pesce, M Soncini, S Deglise, GB Fiore, JA Haefliger, TA Schmidt, MW Majesky, M Sobel, TN Wight
    PLoS ONE, 2018-09-28;13(9):e0204045.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Versican overexpression in human breast cancer lesions: known and new isoforms for stromal tumor targeting.
    Authors: Kischel P, Waltregny D, Dumont B, Turtoi A, Greffe Y, Kirsch S, De Pauw E, Castronovo V
    Int. J. Cancer, 2010-02-01;126(3):640-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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