Human Versican Antibody

Catalog #: MAB3054
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3054
MAB3054-SP

All Versican Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Versican Antibody Summary

Specificity

Detects human Versican in Western blots.

Source

Monoclonal Rat IgG₁ Clone # 255915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Versican

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Versican, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Versican

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Entrez Gene IDs

1462 (Human); 13003 (Mouse)

Alternate Names

Chondroitin sulfate proteoglycan 2WGN; Chondroitin sulfate proteoglycan core protein 2; CSPG2; CSPG2DKFZp686K06110; ERVR; GHAP; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PG-MWGN1; VCAN; versican core protein; versican proteoglycan; Versican

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Versican Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Mechanisms of Hypoxic Up-Regulation of Versican Gene Expression in Macrophages.
Authors: Sotoodehnejadnematalahi F, Staples K, Chrysanthou E, Pearson H, Ziegler-Heitbrock L, Burke B
PLoS ONE, 2015-06-09;10(6):e0125799.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Glomerular endothelial glycocalyx constitutes a barrier to protein permeability.
Authors: Singh A, Satchell SC, Neal CR
J. Am. Soc. Nephrol., 2007-10-17;18(11):2885-93.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot