Human VAP-1/AOC3 Antibody

Catalog #: MAB39571 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB39571
MAB39571-SP

Detection of VAP‑1/AOC3 in HUVEC Human Cells by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VAP-1/AOC3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VAP‑1/AOC3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse VAP‑1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 393106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VAP‑1/AOC3
Gly27-Asn763
Accession # Q16853

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of VAP‑1/AOC3 antibody in HUVEC Human Cells antibody by Flow Cytometry.

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Detection of VAP‑1/AOC3 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VAP-1/AOC3 Monoclonal Antibody (Catalog # MAB39571, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAP-1/AOC3

Vascular adhesion protein-1 (VAP-1) is a copper amine oxidase with a topaquinone cofactor. VAP-1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases (1, 2). VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H₂O₂ (3). The enzyme is sensitive to inhibition by semicarbazide. VAP-1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver (4). VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored (5). The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues (6). VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP-1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent (7). VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases (8).

References

Kurkijärvi, R. et al. (1998) J. Immunol. 161:1549.
Gearing, A.J.H. and W. Newman (1993) Immunol. Today 14:506.
Lizcano, J.M. et al. (1998) Biochem. J. 331:69.
Smith, D.J. et al. (1998) J. Exp. Med. 188:17.
Jaakkala K. et al. (2000) Am. J. Pathol. 157:463.
Salmi, M. and J. Jalkanen (2001) Trends Immunol. 22:211.
Salmi, M. and J. Jalkanen (1996) J. Exp. Med. 183:569.
Dunkel, P. et al. (2008) Curr. Med. Chem. 15:1827.

Long Name

Vascular Adhesion Protein-1

Entrez Gene IDs

8639 (Human); 11754 (Mouse); 29473 (Rat)

Alternate Names

amine oxidase, copper containing 3 (vascular adhesion protein 1); AOC3; Copper amine oxidase; EC 1.4.3; HPAO; HPAOSSAO; Semicarbazide-sensitive amine oxidase; SSAO; VAP1; VAP-1; VAP1EC 1.4.3.21; VAP-1membrane primary amine oxidase; Vascular adhesion protein 1