Human VAMP-5 Antibody

Catalog #: AF7366 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7366
AF7366-SP

1 Image

All VAMP-5 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VAMP-5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VAMP-5 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) VAMP-7 is observed, and less than 1% cross-reactivity with rhVAMP-1, rhVAMP-2, and rhVAMP-8 is observed.

Source

Polyclonal Sheep IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

VAMP-5 in Human Skeletal Muscle. VAMP-5 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Sheep Anti-Human VAMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7366) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: VAMP-5

VAMP-5 (vesicle associated membrane protein 5; also myobrevin) is a member of the synaptobrevin family of proteins. Although its predicted MW is 11 kDa, it runs anomalously at 16 kDa in SDS-Page. VAMP-5 is a type IV transmembrane (TM) protein (i.e.-a type II TM protein whose C-terminus is almost completely transmembrane) that is found in both trans-Golgi and plasma membranes of myotubes (mature skeletal and cardiac muscle cells). While VAMPs are commonly associated with vesicle fusion to t-SNARE in cell membranes, VAMP-5 appears to show no such activity. Human VAMP-5 is 116 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-72) with one vSNARE coiled-coil homology domain, a 21 aa TM segment, and 23 aa C-terminal luminal domain. Over aa 1-72, human VAMP-5 shares 75% aa identity with mouse VAMP-5.

Long Name

Vesicle-Associated Membrane Protein 5

Entrez Gene IDs

10791 (Human); 53620 (Mouse); 89818 (Rat)

Alternate Names

Myobrevin; VAMP5; VAMP-5; vesicle-associated membrane protein 5 (myobrevin); vesicle-associated membrane protein 5