Human ULBP-1 PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1380C
Detection of ULBP‑1 in Jurkat Human Cell Line by Flow Cytometry.
3 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human ULBP-1 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.
Source
Monoclonal Mouse IgG2A Clone # 170818
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ULBP-1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ULBP‑1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human ULBP-1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1380C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of ULBP‑1 in Molt-4 Human Cell Line by Flow Cytometry. Molt-4 human myeloid leukemia cell line was stained with Mouse Anti-Human ULBP-1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1380C, filled histogram) or isotype control antibody (IC003C, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry Detection of Human ULBP-1 by Flow Cytometry View Larger

Detection of Human ULBP-1 by Flow Cytometry Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114
  8. NKG2D and its Ligands, 2002; www.RnDSystems.com.
Long Name
UL16 Binding Protein-1
Entrez Gene IDs
80329 (Human); 77777 (Mouse)
Alternate Names
ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1

Product Datasheets

You must select a language.

x

Citations for Human ULBP-1 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. NKG2A and HLA-E define an alternative immune checkpoint axis in bladder cancer
    Authors: Bérengère Salomé, John P. Sfakianos, Daniel Ranti, Jorge Daza, Christine Bieber, Andrew Charap et al.
    Cancer Cell
  2. Targeting WEE1/AKT restores p53-dependent NK cell activation to induce immune checkpoint blockade responses in 'cold' melanoma
    Authors: SS Dinavahi, YC Chen, K Punnath, A Berg, M Herlyn, M Foroutan, ND Huntington, GP Robertson
    Cancer Immunology Research, 2022-06-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: FACS
  3. Identification of HIV-reservoir cells with reduced susceptibility to antibody-dependent immune response
    Authors: Antonio Astorga-Gamaza, Judith Grau-Expósito, Joaquín Burgos, Jordi Navarro, Adrià Curran, Bibiana Planas et al.
    eLife
  4. Genetic and Molecular Basis of Heterogeneous NK Cell Responses against Acute Leukemia
    Authors: DR Makanga, F Da Rin de, G David, C Willem, L Dubreuil, N Legrand, T Guillaume, P Peterlin, A Lebourgeoi, MC Béné, A Garnier, P Chevallier, K Gendzekhad, A Cesbron, K Gagne, B Clemenceau, C Retière
    Cancers (Basel), 2020-07-16;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor Cells
    Authors: Jaydeep Bhat, Samuel Dubin, Alexandra Dananberg, Elgar Susanne Quabius, Juergen Fritsch, C. Marie Dowds et al.
    Frontiers in Immunology
  6. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function
    Authors: A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard
    BMC Immunol., 2019-01-29;20(1):8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Overcoming Target Driven Fratricide for T Cell Therapy
    Authors: Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al.
    Frontiers in Immunology
  8. Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4? T Cells
    Authors: A Tremblay-M, J Bruneau, B Lebouché, I Lisovsky, R Song, NF Bernard
    Viruses, 2017-10-12;9(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ULBP-1 PerCP-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human ULBP-1 PerCP-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human ULBP-1 PerCP-conjugated Antibody
By Stephane Hua on 07/05/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human